Angel David Paulino-Gonzalez1,2, Hiroshi Sakagami3, Kenjiro Bandow4, Yumiko Kanda4, Yuko Nagasawa4, Yasushi Hibino4, Hiroshi Nakajima4, Satoshi Yokose4, Osamu Amano4, Giichirou Nakaya5, Yukari Koga-Ogawa5, Akiyoshi Shiroto5, Tadamasa Nobesawa5, Daisuke Ueda5, Sachie Nakatani6, Kenji Kobata6, Yosuke Iijima7, Shinsuke Ifuku8, Masaji Yamamoto9, Rene Garcia-Contreras10. 1. Laboratorio de Investigación Interdisciplinaria, Área de Nanoestructuras y Biomateriales, Escuela Nacional de Estudios Superiores Unidad León, León, México. 2. Dental Science, National Autonomous University of Mexico, Mexico City, Mexico. 3. Meikai University School of Dentistry, Saitama, Japan sakagami@dent.meikai.ac.jp dentist.garcia@gmail.com. 4. Meikai University School of Dentistry, Saitama, Japan. 5. Nihon Institute of Medical Science Faculty of Health Sciences, Saitama, Japan. 6. Graduate School of Pharmaceutical Sciences, Josai University, Saitama, Japan. 7. Saitama Medical Center, Saitama Medical University, Saitama, Japan. 8. Department of Veterinary Clinical Medicine, Tottori University, Tottori, Japan. 9. Koyo Chemical Co. Ltd., Tokyo, Japan. 10. Laboratorio de Investigación Interdisciplinaria, Área de Nanoestructuras y Biomateriales, Escuela Nacional de Estudios Superiores Unidad León, León, México sakagami@dent.meikai.ac.jp dentist.garcia@gmail.com.
Abstract
BACKGROUND/AIM: Chitosan-coated iron oxide nanoparticles (Chi-NP) have gained attention because of their biocompatibility, biodegradability, low toxicity and targetability under magnetic field. In this study, we investigated various biological properties of Chi-NP. MATERIALS AND METHODS: Chi-NP was prepared by mixing magnetic NP with chitosan FL-80. Particle size was determined by scanning and transmission electron microscopes, cell viability by MTT assay, cell cycle distribution by cell sorter, synergism with anticancer drugs by combination index, PGE2 production in human gingival fibroblast was assayed by ELISA. RESULTS: The synthetic process of Chi-NP from FL-80 and magnetic NP increased the affinity to cells, up to the level attained by nanofibers. Upon contact with the culture medium, Chi-NP instantly formed aggregates and interfered with intracellular uptake. Aggregated Chi-NP did not show cytotoxicity, synergism with anticancer drugs, induce apoptosis (accumulation of subG1 cell population), protect the cells from X-ray-induced damage, nor affected both basal and IL-1β-induced PGE2 production. CONCLUSION: Chi-NP is biologically inert and shows high affinity to cells, further confirming its superiority as a scaffold for drug delivery. Copyright
BACKGROUND/AIM: Chitosan-coated iron oxide nanoparticles (Chi-NP) have gained attention because of their biocompatibility, biodegradability, low toxicity and targetability under magnetic field. In this study, we investigated various biological properties of Chi-NP. MATERIALS AND METHODS:Chi-NP was prepared by mixing magnetic NP with chitosan FL-80. Particle size was determined by scanning and transmission electron microscopes, cell viability by MTT assay, cell cycle distribution by cell sorter, synergism with anticancer drugs by combination index, PGE2 production in human gingival fibroblast was assayed by ELISA. RESULTS: The synthetic process of Chi-NP from FL-80 and magnetic NP increased the affinity to cells, up to the level attained by nanofibers. Upon contact with the culture medium, Chi-NP instantly formed aggregates and interfered with intracellular uptake. Aggregated Chi-NP did not show cytotoxicity, synergism with anticancer drugs, induce apoptosis (accumulation of subG1 cell population), protect the cells from X-ray-induced damage, nor affected both basal and IL-1β-induced PGE2 production. CONCLUSION:Chi-NP is biologically inert and shows high affinity to cells, further confirming its superiority as a scaffold for drug delivery. Copyright