Zhengkun Zhang1, Yang Lu1, Wenjing Xu1, Li Sui1, Qian Du1, Yangzhou Wang1, Yu Zhao1, Qiyun Li2. 1. Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture, Changchun, 130033, Jilin Province, P. R. China. 2. Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture, Changchun, 130033, Jilin Province, P. R. China. qyli@cjaas.com.
Abstract
BACKGROUND: Beauveria bassiana (B. bassiana) is a famous entomopathogenic fungus that could parasitize on hundreds of insect species, which are being used as an environmentally friendly mycoinsecticide. Nevertheless, the possible effect of genetic diversity of these B. bassiana isolates from different hosts on virulence has not been explored before. In order to explore that issue, we compared the genome sequences among seventeen B. bassiana isolates from 17 different insects using whole genome re-sequencing, with B. bassiana strain ARSEF 2860 as the reference genome. RESULTS: There were a total of 10,098 missense mutated genes, 720 positively selected genes were identified in 17 strains of B. bassiana. Among these, two genes with high frequency mutations encode the toxin-producing non-ribosomal peptide synthase (NRPS) protein. Seven genes undergoing positive selection were enriched in the two-component signaling pathway that is known to regulate the fungal toxicity. In addition, the domain changes of three positively selected genes are also directly related to the virulence plasticity. Besides, the functional categorization of mutated genes showed that most of them involved in the biological functions of toxic proteins involved in. CONCLUSIONS: Based on our data, our results indicate that several mutated genes and positively selected genes may underpin virulence of B. bassiana towards hosts during infection process, which provide an insight into the potential effects of natural variation on the virulence of B. bassiana, which will be useful in screening out potential virulence factors in B. bassiana.
BACKGROUND:Beauveria bassiana (B. bassiana) is a famous entomopathogenic fungus that could parasitize on hundreds of insect species, which are being used as an environmentally friendly mycoinsecticide. Nevertheless, the possible effect of genetic diversity of these B. bassiana isolates from different hosts on virulence has not been explored before. In order to explore that issue, we compared the genome sequences among seventeen B. bassiana isolates from 17 different insects using whole genome re-sequencing, with B. bassiana strain ARSEF 2860 as the reference genome. RESULTS: There were a total of 10,098 missense mutated genes, 720 positively selected genes were identified in 17 strains of B. bassiana. Among these, two genes with high frequency mutations encode the toxin-producing non-ribosomal peptide synthase (NRPS) protein. Seven genes undergoing positive selection were enriched in the two-component signaling pathway that is known to regulate the fungal toxicity. In addition, the domain changes of three positively selected genes are also directly related to the virulence plasticity. Besides, the functional categorization of mutated genes showed that most of them involved in the biological functions of toxic proteins involved in. CONCLUSIONS: Based on our data, our results indicate that several mutated genes and positively selected genes may underpin virulence of B. bassiana towards hosts during infection process, which provide an insight into the potential effects of natural variation on the virulence of B. bassiana, which will be useful in screening out potential virulence factors in B. bassiana.
Authors: Karen A O'Hanlon; Timothy Cairns; Deirdre Stack; Markus Schrettl; Elaine M Bignell; Kevin Kavanagh; Sinéad M Miggin; Grainne O'Keeffe; Thomas O Larsen; Sean Doyle Journal: Infect Immun Date: 2011-07-11 Impact factor: 3.441
Authors: Annemarie Wehenkel; Marco Bellinzoni; Martin Graña; Rosario Duran; Andrea Villarino; Pablo Fernandez; Gwénaëlle Andre-Leroux; Patrick England; Howard Takiff; Carlos Cerveñansky; Stewart T Cole; Pedro M Alzari Journal: Biochim Biophys Acta Date: 2007-08-14
Authors: Daniel E Voth; Dale Howe; Paul A Beare; Joseph P Vogel; Nathan Unsworth; James E Samuel; Robert A Heinzen Journal: J Bacteriol Date: 2009-05-01 Impact factor: 3.490
Authors: Eva Arrebola; Francisco M Cazorla; Diego Romero; Alejandro Pérez-García; Antonio de Vicente Journal: Mol Plant Microbe Interact Date: 2007-05 Impact factor: 4.171
Authors: Ken Chen; John W Wallis; Michael D McLellan; David E Larson; Joelle M Kalicki; Craig S Pohl; Sean D McGrath; Michael C Wendl; Qunyuan Zhang; Devin P Locke; Xiaoqi Shi; Robert S Fulton; Timothy J Ley; Richard K Wilson; Li Ding; Elaine R Mardis Journal: Nat Methods Date: 2009-08-09 Impact factor: 28.547