Tsehayneh Kelemu1, Lena Erlandsson2, Daniel Seifu3, Eva Hansson4, Markos Abebe5, Sisay Teklu6, Selfu Girma7, James A Traherne8, Ashley Moffett9, Stefan R Hansson10. 1. Department of Biochemistry, College of Health Sciences, Addis Ababa University, Ethiopia. Electronic address: tkelemu_2005@yahoo.com. 2. Department of Obstetrics and Gynecology, Institute of Clinical Sciences Lund, Lund University, Sweden. Electronic address: lena.erlandsson@med.lu.se. 3. Department of Biochemistry, College of Health Sciences, Addis Ababa University, Ethiopia; Department of Biochemistry, Division of Biomedical Sciences, University of Global Health Equity, Rwanda. Electronic address: abbysinia2002@gmail.com. 4. Department of Obstetrics and Gynecology, Institute of Clinical Sciences Lund, Lund University, Sweden. Electronic address: eva.hansson@med.lu.se. 5. Armauer Hanson Research Institute, Ethiopia. Electronic address: markosabebe@yahoo.com. 6. Department of Obstetrics and Gynecology, College of Health Sciences, Addis Ababa University, Ethiopia. Electronic address: siteet@yahoo.com. 7. Armauer Hanson Research Institute, Ethiopia. Electronic address: selfugirma2@gmail.com. 8. Department of Pathology, University of Cambridge, Cambridge, UK. Electronic address: jat51@cam.ac.uk. 9. Department of Pathology, University of Cambridge, Cambridge, UK. Electronic address: am485@cam.ac.uk. 10. Department of Obstetrics and Gynecology, Institute of Clinical Sciences Lund, Lund University, Sweden. Electronic address: stefan.hansson@med.lu.se.
Abstract
INTRODUCTION: Preeclampsia (PE) is a human specific pregnancy-related syndrome of unknown etiology that affects 2-8 % of pregnancies. Polymorphism in maternal Killer Cell Immunoglobulin-like Receptors (KIRs) and the ligand fetal Human Leukocyte Antigen-C (HLA-C) may predispose pregnant mothers for PE due to defective trophoblast invasion into the maternal decidua. Our study aimed to investigate the association between maternal KIR and fetal HLA-C polymorphism and PE in Ethiopian pregnant women. METHODS: We included a total of 288 (157 controls and 131 PE cases) in a case-controls study at Adama Regional Referral Hospital, Ethiopia. The KIR and HLA-C genotyping was done using traditional polymerase chain reaction on genomic DNA extracted form maternal venous and cord blood followed by 2% agarose gel electrophoresis. RESULTS: The statistical associations between variables were evaluated using Pearson's Chi-square test. P < 0.05, with 95 % confidence interval was considered statistically significant. A significant association was observed between the KIR2DS1 and PE, with a higher frequency (60.5 %) of the gene in the control group. Similarly, a significant association was observed between KIR AA genotype and PE, with a higher frequency (38.2 %) of this genotype in the PE group. Ethiopians share the same risk genotype for PE as seen in previous African and European studies, namely homozygosity of a maternal KIR AA genotype. However, Ethiopians differ from other East African populations by sharing the same protective KIR2DS1 gene as Europeans.
INTRODUCTION: Preeclampsia (PE) is a human specific pregnancy-related syndrome of unknown etiology that affects 2-8 % of pregnancies. Polymorphism in maternal Killer Cell Immunoglobulin-like Receptors (KIRs) and the ligand fetal Human Leukocyte Antigen-C (HLA-C) may predispose pregnant mothers for PE due to defective trophoblast invasion into the maternal decidua. Our study aimed to investigate the association between maternal KIR and fetal HLA-C polymorphism and PE in Ethiopian pregnant women. METHODS: We included a total of 288 (157 controls and 131 PE cases) in a case-controls study at Adama Regional Referral Hospital, Ethiopia. The KIR and HLA-C genotyping was done using traditional polymerase chain reaction on genomic DNA extracted form maternal venous and cord blood followed by 2% agarose gel electrophoresis. RESULTS: The statistical associations between variables were evaluated using Pearson's Chi-square test. P < 0.05, with 95 % confidence interval was considered statistically significant. A significant association was observed between the KIR2DS1 and PE, with a higher frequency (60.5 %) of the gene in the control group. Similarly, a significant association was observed between KIR AA genotype and PE, with a higher frequency (38.2 %) of this genotype in the PE group. Ethiopians share the same risk genotype for PE as seen in previous African and European studies, namely homozygosity of a maternal KIR AA genotype. However, Ethiopians differ from other East African populations by sharing the same protective KIR2DS1 gene as Europeans.
Authors: Min Xie; Yan Li; Yi-Zi Meng; Peng Xu; Yong-Guang Yang; Shuai Dong; Jin He; Zheng Hu Journal: Front Immunol Date: 2022-06-01 Impact factor: 8.786
Authors: Tsehayneh Kelemu; Lena Erlandsson; Daniel Seifu; Markos Abebe; Sisay Teklu; Jill R Storry; Stefan R Hansson Journal: Int J Mol Sci Date: 2020-08-14 Impact factor: 5.923