Shuhao Huo1, Junzhi Liu2, Feifei Zhu3, Sajid Basheer4, David Necas5, Renchuan Zhang5, Kun Li6, Dongjie Chen5, Pengfei Cheng7, Krik Cobb5, Paul Chen5, Bailey Brandel5, Roger Ruan8. 1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, MN 55108, United States. 2. College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China. 3. Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China. 4. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. 5. Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, MN 55108, United States. 6. School of Resources, Environmental & Chemical Engineering, Nanchang University, Nanchang 330047, China. 7. Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, MN 55108, United States; College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, Zhejiang 315211, China. 8. Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, MN 55108, United States. Electronic address: ruanx001@umn.edu.
Abstract
A weak electric field (EF) was applied to decolorize the swine anaerobic effluent, which was followed by N:P ratio adjustment via intermittent-vacuum stripping (IVS) system for oil-rich filamentous microalgae Tribonema sp. cultivation. A higher electric field strength, higher temperature, and lower pH conditions showed higher efficiency in decolorization and nutrients removal during EF application. In the group of 30:1 (N:P) ratio, Tribonema sp. had the largest biomass accumulation (2.04 g·L-1) after 14 days cultivation. However, the 20:1 group had highest oil accumulation (oil content 55.4 ± 3.4%), while 30:1 (N: P) group was 42.3 ± 1.8%. Under the conditions of sufficient nitrogen (50:1 group), the highest contents of α-linolenic acid (15.5%) and ω-3 fatty acids (21.8%) were reached. The integrated treatment of EF, IVS and microalgae cultivation demonstrated to be effective for nutrients recycling and sustainable biomass production.
A weak electric field (EF) was applied to decolorize the n class="Species">swine anaerobic effluent, which was followed by N:P ratio adjustment via inpan>termittent-vacuum strippinpan>g (IVS) system for n class="Disease">oil-rich filamentous microalgae Tribonema sp. cultivation. A higher electric field strength, higher temperature, and lower pH conditions showed higher efficiency in decolorization and nutrients removal during EF application. In the group of 30:1 (N:P) ratio, Tribonema sp. had the largest biomass accumulation (2.04 g·L-1) after 14 days cultivation. However, the 20:1 group had highest oil accumulation (oil content 55.4 ± 3.4%), while 30:1 (N: P) group was 42.3 ± 1.8%. Under the conditions of sufficient nitrogen (50:1 group), the highest contents of α-linolenic acid (15.5%) and ω-3 fatty acids (21.8%) were reached. The integrated treatment of EF, IVS and microalgae cultivation demonstrated to be effective for nutrients recycling and sustainable biomass production.