| Literature DB >> 32594407 |
Thomas Bocklitz1, Anja Silge2, Hyeonsoo Bae2, Marko Rodewald2, Fisseha Bekele Legesse2, Tobias Meyer2, Jürgen Popp3.
Abstract
In this chapter, we will introduce and review molecular-sensitive imaging techniques, which close the gap between ex vivo and in vivo analysis. In detail, we will introduce spontaneous Raman spectral imaging, coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS), second-harmonic generation (SHG) and third-harmonic generation (THG), two-photon excited fluorescence (TPEF), and fluorescence lifetime imaging (FLIM). After reviewing these imaging techniques, we shortly introduce chemometric methods and machine learning techniques, which are needed to use these imaging techniques in diagnostic applications.Entities:
Keywords: Chemometrics; Coherent anti-Stokes Raman scattering (CARS); Fluorescence lifetime imaging (FLIM); Machine learning; Non-invasive imaging; Raman spectral imaging; Second-harmonic generation (SHG); Stimulated Raman scattering (SRS); Third-harmonic generation (THG); Two-photon excited fluorescence (TPEF)
Mesh：
Year: 2020 PMID： 32594407 DOI： 10.1007/978-3-030-42618-7_25
Source DB: PubMed Journal: Recent Results Cancer Res ISSN： 0080-0015