Christopher A P Batho1,2, Richard J Mills1, James E Hudson3. 1. QIMR Berghofer Medical Research Institute, 300 Herston Rd, Brisbane, Queensland, 4006, Australia. 2. School of Biomedical Sciences, Queensland University of Technology, Brisbane, Queensland, Australia. 3. QIMR Berghofer Medical Research Institute, 300 Herston Rd, Brisbane, Queensland, 4006, Australia. James.Hudson@QIMRBerghofer.edu.au.
Abstract
PURPOSE OF REVIEW: This review summarizes the important role that metabolism plays in driving maturation of human pluripotent stem cell-derived cardiomyocytes. RECENT FINDINGS: Human pluripotent stem cell-derived cardiomyocytes provide a model system for human cardiac biology. However, these models have been unable to fully recapitulate the maturity observed in the adult heart. By simulating the glucose to fatty acid transition observed in neonatal mammals, human pluripotent stem cell-derived cardiomyocytes undergo structural and functional maturation also accompanied by transcriptional changes and cell cycle arrest. The role of metabolism in energy production, signaling, and epigenetic modifications illustrates that metabolism and cellular phenotype are intimately linked. Further understanding of key metabolic factors driving cardiac maturation will facilitate the generation of more mature human pluripotent stem cell-derived cardiomyocyte models. This will increase our understanding of cardiac biology and potentially lead to novel therapeutics to enhance heart function.
PURPOSE OF REVIEW: This review summarizes the important role that metabolism plays in driving maturation of human pluripotent stem cell-derived cardiomyocytes. RECENT FINDINGS:Human pluripotent stem cell-derived cardiomyocytes provide a model system for human cardiac biology. However, these models have been unable to fully recapitulate the maturity observed in the adult heart. By simulating the glucose to fatty acid transition observed in neonatal mammals, human pluripotent stem cell-derived cardiomyocytes undergo structural and functional maturation also accompanied by transcriptional changes and cell cycle arrest. The role of metabolism in energy production, signaling, and epigenetic modifications illustrates that metabolism and cellular phenotype are intimately linked. Further understanding of key metabolic factors driving cardiac maturation will facilitate the generation of more mature human pluripotent stem cell-derived cardiomyocyte models. This will increase our understanding of cardiac biology and potentially lead to novel therapeutics to enhance heart function.
Authors: Martha E Floy; Fathima Shabnam; Aaron D Simmons; Vijesh J Bhute; Gyuhyung Jin; Will A Friedrich; Alexandra B Steinberg; Sean P Palecek Journal: Annu Rev Chem Biomol Eng Date: 2022-03-23 Impact factor: 9.700
Authors: Jolanda van der Velden; Birgit Goversen; Sofija Vučković; Rafeeh Dinani; Edgar E Nollet; Diederik W D Kuster; Jan Willem Buikema; Riekelt H Houtkooper; Miranda Nabben Journal: Stem Cell Res Ther Date: 2022-07-23 Impact factor: 8.079