Raphaela Costa Ferreira1, Marilene Brandão Tenório Fragoso2, Micaely Cristina Dos Santos Tenório3, Amylly Sanuelly da Paz Martins2, Alexandre Urban Borbely1, Fabiana Andréa Moura4, Marília Oliveira Fonseca Goulart5, Alane Cabral Menezes de Oliveira6. 1. Pós-Graduação em Ciências da Saúde/Universidade Federal de Alagoas (ICBS/UFAL), Brazil. 2. Instituto de Química e Biotecnologia (IQB/UFAL), Rede Nordeste de Biotecnologia (RENORBIO), Brazil. 3. Instituto de Química e Biotecnologia (IQB/UFAL), Rede Nordeste de Biotecnologia (RENORBIO), Brazil; Pós-Graduação em Nutrição/Universidade Federal de Alagoas (FANUT/UFAL), Brazil. 4. Pós-Graduação em Nutrição/Universidade Federal de Alagoas (FANUT/UFAL), Brazil; Docente da Faculdade de Nutrição/ Universidade Federal de Alagoas (FANUT/UFAL), Brazil; Pós-Graduação em Ciências Médicas/Universidade Federal de Alagoas (PPGCM/UFAL), Brazil. 5. Pós-Graduação em Ciências da Saúde/Universidade Federal de Alagoas (ICBS/UFAL), Brazil; Instituto de Química e Biotecnologia (IQB/UFAL), Rede Nordeste de Biotecnologia (RENORBIO), Brazil. 6. Pós-Graduação em Nutrição/Universidade Federal de Alagoas (FANUT/UFAL), Brazil; Docente da Faculdade de Nutrição/ Universidade Federal de Alagoas (FANUT/UFAL), Brazil. Electronic address: alanecabral@gmail.com.
Abstract
OBJECTIVE: To compare redox and inflammatory markers between normal and PE-derived placentas and to evaluate the relationship between placental redox imbalance markers and perinatal outcomes in pregnancies with PE. METHODS: This was a cross-sectional study conducted at the maternity hospital of a university hospital in Maceio-Alagoas, Brazil, in 2017, including women diagnosed with PE and healthy pregnant women and their conceptuses. After screening, standardized questionnaires containing socioeconomic, clinical, obstetric and anthropometric data were applied. After delivery, placental samples were collected for quantification of biomarkers of redox imbalance (catalase - CAT; malondialdehyde - MDA; hydrogen peroxide - H2O2; superoxide dismutase - SOD; reduced glutathione - GSH; oxidized glutathione - GSSG; and their ratio - GSH/GSSG) and inflammation (myeloperoxidase - MPO; interleukin (IL)-6; IL-8; IL-10; and tumor necrosis factor-alpha - TNF-α). All biomarkers were evaluated via linear regression with adjustments of variables with measures of weight, length, head circumference (HC), chest circumference (CC) and gestational age of newborns at birth, considering p < 0.05 as significant. RESULTS: A total of 100 pregnant women with PE and 50 healthy pregnant women were studied. Higher placental levels of catalase (p = 0.018), SOD (p = 0.031), the GSH/GSSG ratio (p = 0.019) and IL-6 (p = 0.010) and lower GSSG (p = 0.001) were observed in pregnant women with PE than in the control group. Positive associations between placental GSH levels and body weight, HC, CC and gestational age at birth (p < 0.05) were identified. CONCLUSION: PE-derived placentas had high concentrations of some antioxidants (enzymes and thiols), which might be a compensation mechanism against oxidative stress. Placental GSH levels were the only biomarker, among the studied ones, related positively with beneficial perinatal outcomes, suggesting that this endogenous antioxidant plays an important role in maintaining the health of the conceptus and women with PE.
OBJECTIVE: To compare redox and inflammatory markers between normal and PE-derived placentas and to evaluate the relationship between placental redox imbalance markers and perinatal outcomes in pregnancies with PE. METHODS: This was a cross-sectional study conducted at the maternity hospital of a university hospital in Maceio-Alagoas, Brazil, in 2017, including women diagnosed with PE and healthy pregnant women and their conceptuses. After screening, standardized questionnaires containing socioeconomic, clinical, obstetric and anthropometric data were applied. After delivery, placental samples were collected for quantification of biomarkers of redox imbalance (catalase - CAT; malondialdehyde - MDA; hydrogen peroxide - H2O2; superoxide dismutase - SOD; reduced glutathione - GSH; oxidized glutathione - GSSG; and their ratio - GSH/GSSG) and inflammation (myeloperoxidase - MPO; interleukin (IL)-6; IL-8; IL-10; and tumor necrosis factor-alpha - TNF-α). All biomarkers were evaluated via linear regression with adjustments of variables with measures of weight, length, head circumference (HC), chest circumference (CC) and gestational age of newborns at birth, considering p < 0.05 as significant. RESULTS: A total of 100 pregnant women with PE and 50 healthy pregnant women were studied. Higher placental levels of catalase (p = 0.018), SOD (p = 0.031), the GSH/GSSG ratio (p = 0.019) and IL-6 (p = 0.010) and lower GSSG (p = 0.001) were observed in pregnant women with PE than in the control group. Positive associations between placental GSH levels and body weight, HC, CC and gestational age at birth (p < 0.05) were identified. CONCLUSION: PE-derived placentas had high concentrations of some antioxidants (enzymes and thiols), which might be a compensation mechanism against oxidative stress. Placental GSH levels were the only biomarker, among the studied ones, related positively with beneficial perinatal outcomes, suggesting that this endogenous antioxidant plays an important role in maintaining the health of the conceptus and women with PE.
Authors: Saghi Zafaranieh; Anna M Dieberger; Barbara Leopold-Posch; Berthold Huppertz; Sebastian Granitzer; Markus Hengstschläger; Claudia Gundacker; Gernot Desoye; Mireille N M van Poppel Journal: Biomedicines Date: 2022-05-05
Authors: Samprikta Manna; Camino S M Ruano; Jana-Charlotte Hegenbarth; Daniel Vaiman; Shailendra Gupta; Fergus P McCarthy; Céline Méhats; Cathal McCarthy; Clara Apicella; Julia Scheel Journal: Antioxidants (Basel) Date: 2022-03-18