Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Comparison of two dimensional immunoelectrophoresis and multimer analysis in the study of von Willebrand factor.

Literature DB >> 3258872

Comparison of two dimensional immunoelectrophoresis and multimer analysis in the study of von Willebrand factor.

Abstract

To assess the validity of the multimeric analysis of von Willebrand factor (vWf), this technique was compared with two dimensional immunoelectrophoresis using samples of purified vWf obtained by gel filtration and plasma samples from a patient with severe von Willebrands disease who was receiving prophylaxis with cryoprecipitate. It is concluded that except for specialised clinical and research purposes, two dimensional immunoelectrophoresis provides a clear picture of the multimeric composition of the vWf molecule, and that this is sufficient for routine research and clinical use, without having to confirm the data by multimeric analysis.

Entities: Disease Gene Species

Mesh：

Substances：
von Willebrand Factor

Year: 1988 PMID： 3258872 PMCID： PMC1141438 DOI： 10.1136/jcp.41.3.346

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

24 in total

1. Determination of the von Willebrand's disease antigen (factor VIII-related antigen) in plasma by quantitative immunoelectrophoresis.

Authors: T S Zimmerman; L W Hoyer; L Dickson; T S Edgington
Journal: J Lab Clin Med Date: 1975-07

2. Letter: A method for assaying von Willebrand factor (ristocetin cofactor).

Authors: D E Macfarlane; J Stibbe; E P Kirby; M B Zucker; R A Grant; J McPherson
Journal: Thromb Diath Haemorrh Date: 1975-09-30

3. MEASUREMENT OF PLATELET ADHESIVENESS. A SIMPLE IN VITRO TECHNIQUE DEMONSTRATING AN ABNORMALITY IN VON WILLEBRAND'S DISEASE.

Authors: E W SALZMAN
Journal: J Lab Clin Med Date: 1963-11

4. A two-site, monoclonal antibody-based immunoassay for von Willebrand factor--demonstration that vWF function resides in a conformational epitope.

Authors: S Chand; A McCraw; R Hutton; E G Tuddenham; A H Goodall
Journal: Thromb Haemost Date: 1986-06-30 Impact factor: 5.249

5. Proteolytic cleavage of human von Willebrand factor induced by enzyme(s) released from polymorphonuclear cells.

Authors: E A Thompson; M A Howard
Journal: Blood Date: 1986-05 Impact factor: 22.113

6. A new variant of dominant type II von Willebrand's disease with aberrant multimeric pattern of factor VIII-related antigen (type IID).

Authors: S Kinoshita; J Harrison; J Lazerson; C F Abildgaard
Journal: Blood Date: 1984-06 Impact factor: 22.113

7. Human von Willebrand factor: a multivalent protein composed of identical subunits.

Authors: M W Chopek; J P Girma; K Fujikawa; E W Davie; K Titani
Journal: Biochemistry Date: 1986-06-03 Impact factor: 3.162

8. The agglutination of human platelets by botrocetin: evidence that botrocetin and ristocetin act at different sites on the factor VIII molecule and platelet membrane.

Authors: M A Howard; J Perkin; H H Salem; B G Firkin
Journal: Br J Haematol Date: 1984-05 Impact factor: 6.998