José Morales-Roselló1,2, José Luis García-Giménez3,4, Llucia Martinez Priego5, Daymé González-Rodríguez3, Salvador Mena-Mollá3,4, Angel Maquieira Catalá6, Gabriela Loscalzo7, Silvia Buongiorno7, Vaidile Jakaite7, Antonio José Cañada Martínez8, Alfredo Perales Marín7,9. 1. Servicio de Obstetricia, Hospital Universitario y Politécnico La Fe, Valencia, Spain, jose.morales@uv.es. 2. Departamento de Pediatría, Obstetricia y Ginecología, Universidad de Valencia, Valencia, Spain, jose.morales@uv.es. 3. EpiDisease SL, and Consortium Center for Biomedical Network Research on Rare Diseases (CIBERER), Institute of Health Carlos III, Valencia, Spain. 4. Departamento de Fisiología, Universidad de Valencia, Valencia, Spain. 5. Servicio de Secuenciación, Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunidad valenciana (FISABIO), Valencia, Spain. 6. Departamento de Química, Universidad Politécnica de Valencia, Valencia, Spain. 7. Servicio de Obstetricia, Hospital Universitario y Politécnico La Fe, Valencia, Spain. 8. Unidad de Bioestadística, Instituto de Investigación Sanitaria La Fe, Valencia, Spain. 9. Departamento de Pediatría, Obstetricia y Ginecología, Universidad de Valencia, Valencia, Spain.
Abstract
OBJECTIVE: It was the aim of this study to describe a micro-RNA (miRNA) profile characteristic of late-onset fetal growth restriction (FGR) and to investigate the pathways involved in their biochemical action. METHODS: In this prospective study, 25 fetuses (16 normal and 9 with FGR [estimated fetal weight <10th centile plus cerebroplacental ratio <0.6765 multiples of the median]) were evaluated with Doppler ultrasound after 36 weeks. Afterwards, for every fetus, plasma from umbilical vein blood was collected at birth, miRNA was extracted, and full miRNA sequencing was performed. Subsequently, comparisons were done in order to obtain those miRNAs that were differentially expressed. RESULTS: The FGR fetuses expressed upregulation of two miRNAs: miR-25-3p and, especially, miR-148b-3p, a miRNA directly involved in Schwann cell migration, neuronal plasticity, and energy metabolism (p = 0.0072, p = 0.0013). CONCLUSIONS: FGR fetuses express a different miRNA profile, which includes overexpression of miR-25-3p and miR-148b-3p. This information might improve our understanding of the pathophysiological processes involved in late-onset FGR. Future validation and feasibility studies will be required to propose miRNAs as a valid tool in the diagnosis and management of FGR.
OBJECTIVE: It was the aim of this study to describe a micro-RNA (miRNA) profile characteristic of late-onset fetal growth restriction (FGR) and to investigate the pathways involved in their biochemical action. METHODS: In this prospective study, 25 fetuses (16 normal and 9 with FGR [estimated fetal weight <10th centile plus cerebroplacental ratio <0.6765 multiples of the median]) were evaluated with Doppler ultrasound after 36 weeks. Afterwards, for every fetus, plasma from umbilical vein blood was collected at birth, miRNA was extracted, and full miRNA sequencing was performed. Subsequently, comparisons were done in order to obtain those miRNAs that were differentially expressed. RESULTS: The FGR fetuses expressed upregulation of two miRNAs: miR-25-3p and, especially, miR-148b-3p, a miRNA directly involved in Schwann cell migration, neuronal plasticity, and energy metabolism (p = 0.0072, p = 0.0013). CONCLUSIONS: FGR fetuses express a different miRNA profile, which includes overexpression of miR-25-3p and miR-148b-3p. This information might improve our understanding of the pathophysiological processes involved in late-onset FGR. Future validation and feasibility studies will be required to propose miRNAs as a valid tool in the diagnosis and management of FGR.
Authors: Gabriela Loscalzo; Julia Scheel; José Santiago Ibañez-Cabellos; Eva García-Lopez; Shailendra Gupta; José Luis García-Gimenez; Salvador Mena-Mollá; Alfredo Perales-Marín; José Morales-Roselló Journal: Int J Mol Sci Date: 2021-12-28 Impact factor: 5.923