Sajjad Biglari1, Koorosh Kamali2, Sousan Banihashemi2, Arash Faal Sezavari1, Seyed Mohsen Aghajanpour-Mir1,3, Farkhondeh Behjati1. 1. Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran. 2. Department of Public Health, School of Public Health, Zanjan University of Medical Sciences. 3. Cellular & Molecular Biology Research Center, Health Institute, Babol University of Medical Sciences, Babol, Iran.
Abstract
BACKGROUND: Alzheimer's disease is one of the most common neurodegenerative and dementia disorders in people between the ages of 30 and 65. When symptoms appear in this age group, the disease is referred to as early-onset Alzheimer's disease (EOAD). Unfortunately, the symptoms are progressive and no current treatments are effective. METHODS: In this research, 13 patients, aged 37 to 65 years with symptoms of early-onset Alzheimer's disease, were studied. First, patient lymphocytes were isolated and cultured in RPMI 1640 medium using a special micronucleus (MN) culture method. Next, the lymphocytes were harvested and prepared on slides. The slides were then examined by fluorescent microscopy using a unique FISH protocol specific for MNs. The patients were divided into groups aged 30-39, 40-49, and 50-65. RESULTS: We found that 19.76% of the MNs from our EOAD patients originated in chromosome 21. Micronuclei originated in chromosome 21 in 21.20 and 16.52% of patients without and with family histories of Alzheimer's, respectively. This difference was not significant. Also, the percentage of micronuclei originating in chromosome 21 was not dependent on the patient age at the time of the study, or symptom onset age or duration. CONCLUSION: This study shows that the rate of micronuclei with the origin of chromosome 21 is high in these patients. However, the micronucleus increased has no significant relationship with age and duration of disease or family history of it.
BACKGROUND: Alzheimer's disease is one of the most common neurodegenerative and dementia disorders in people between the ages of 30 and 65. When symptoms appear in this age group, the disease is referred to as early-onset Alzheimer's disease (EOAD). Unfortunately, the symptoms are progressive and no current treatments are effective. METHODS: In this research, 13 patients, aged 37 to 65 years with symptoms of early-onset Alzheimer's disease, were studied. First, patient lymphocytes were isolated and cultured in RPMI 1640 medium using a special micronucleus (MN) culture method. Next, the lymphocytes were harvested and prepared on slides. The slides were then examined by fluorescent microscopy using a unique FISH protocol specific for MNs. The patients were divided into groups aged 30-39, 40-49, and 50-65. RESULTS: We found that 19.76% of the MNs from our EOAD patients originated in chromosome 21. Micronuclei originated in chromosome 21 in 21.20 and 16.52% of patients without and with family histories of Alzheimer's, respectively. This difference was not significant. Also, the percentage of micronuclei originating in chromosome 21 was not dependent on the patient age at the time of the study, or symptom onset age or duration. CONCLUSION: This study shows that the rate of micronuclei with the origin of chromosome 21 is high in these patients. However, the micronucleus increased has no significant relationship with age and duration of disease or family history of it.
Authors: X Zhu; S L Siedlak; Y Wang; G Perry; R J Castellani; M L Cohen; M A Smith Journal: Neuropathol Appl Neurobiol Date: 2007-11-11 Impact factor: 8.090
Authors: F Trippi; N Botto; R Scarpato; L Petrozzi; U Bonuccelli; S Latorraca; S Sorbi; L Migliore Journal: Mutagenesis Date: 2001-07 Impact factor: 3.000
Authors: A Goate; M C Chartier-Harlin; M Mullan; J Brown; F Crawford; L Fidani; L Giuffra; A Haynes; N Irving; L James Journal: Nature Date: 1991-02-21 Impact factor: 49.962
Authors: L Petrozzi; C Lucetti; R Scarpato; G Gambaccini; F Trippi; S Bernardini; P Del Dotto; L Migliore; U Bonuccelli Journal: Neurol Sci Date: 2002-09 Impact factor: 3.307
Authors: Vladan P Bajić; Biljana Spremo-Potparević; Lada Zivković; David J Bonda; Sandra L Siedlak; Gemma Casadesus; Hyoung-Gon Lee; Mark A Smith Journal: Med Hypotheses Date: 2009-07-31 Impact factor: 1.538