Azza Gaber Antar Farag1, Shymaa A El Askary2, Waleed M Fathy3, Fathia Elbassal3, Ayman Ali Azzam4, Nermin Reda Tayel5, Samah Saad Abdul Karim6, Wafaa Ahmed Shehata1. 1. Dermatology, Andrology & STDs Department, Faculty of Medicine, Menoufia University, Al Menoufia, Egypt. 2. Medical Microbiology and Immunology Department, Faculty of Medicine, Menoufia University, Minufya, Egypt. 3. Clinical Pathology Department, Faculty of Medicine, Menoufia University, Minufya, Egypt. 4. Clinical Biochemistry Department, National Liver Institute, Menoufia University, Minufya, Egypt. 5. Department of Molecular Diagnostics and Therapeutics, Genetic Engineering Biotechnology Research Institute, Sadat City, Egypt. 6. Dermatology Department, Shebin ElKom Teaching Hospital, Minufya, Egypt.
Abstract
BACKGROUND: Leprosy is a chronic contagious disease caused by Mycobacterium lepraea. CD163 is a monocyte trans-membrane glycoprotein receptor (mCD163) that sheds from the cell surface and circulates as a soluble (serum) form (sCD163). Changes in the mCD163 and sCD163 levels could mirror the categorization of inflammatory procedure, demonstrating a possible use of CD163 as a diagnostic indicator of inflammation. OBJECTIVE: To investigate the possible role of CD163 (sCD163 and mCD163) in leprosy pathogenesis and to assess whether CD163 is a helpful inflammatory marker of leprosy development and typing. PATIENTS AND METHODS: This case control study included 70 leprosy patients and 30 healthy controls. Leprosy patients were classified according to the Madrid criteria (1953) into: tuberculoid leprosy (TT), border-line leprosy (BL), and lepromatous leprosy (LL). For all participants, complete blood count (CBC), serum CD163 using ELISA and monocytes positive for CD163 using flow cytometry were done. RESULTS: Leprosy patients had significantly low WBCs and platelet counts (p<0.001) and had significantly higher sCD163 (p=0.025) and mCD163 (p=0.042) that were highest in LL followed by BL, then TT patients (p<0.001). There was a significant positive correlation between mCD163 and sCD163 levels in leprosy patients (r=0.896, p<0.001). ROC analysis revealed a significant role of serum sCD163 and of mCD163 positive monocytes in the detection (p<0.001) and typing of leprosy (p=0.002 and p<0.001, respectively). CONCLUSION: Both sCD163 and mCD163 positive monocytes may have an active role in leprosy pathogenesis. They could be potential biomarkers for leprosy detection and typing.
BACKGROUND: Leprosy is a chronic contagious disease caused by Mycobacterium lepraea. CD163 is a monocyte trans-membrane glycoprotein receptor (mCD163) that sheds from the cell surface and circulates as a soluble (serum) form (sCD163). Changes in the mCD163 and sCD163 levels could mirror the categorization of inflammatory procedure, demonstrating a possible use of CD163 as a diagnostic indicator of inflammation. OBJECTIVE: To investigate the possible role of CD163 (sCD163 and mCD163) in leprosy pathogenesis and to assess whether CD163 is a helpful inflammatory marker of leprosy development and typing. PATIENTS AND METHODS: This case control study included 70 leprosy patients and 30 healthy controls. Leprosy patients were classified according to the Madrid criteria (1953) into: tuberculoid leprosy (TT), border-line leprosy (BL), and lepromatous leprosy (LL). For all participants, complete blood count (CBC), serum CD163 using ELISA and monocytes positive for CD163 using flow cytometry were done. RESULTS: Leprosy patients had significantly low WBCs and platelet counts (p<0.001) and had significantly higher sCD163 (p=0.025) and mCD163 (p=0.042) that were highest in LL followed by BL, then TT patients (p<0.001). There was a significant positive correlation between mCD163 and sCD163 levels in leprosy patients (r=0.896, p<0.001). ROC analysis revealed a significant role of serum sCD163 and of mCD163 positive monocytes in the detection (p<0.001) and typing of leprosy (p=0.002 and p<0.001, respectively). CONCLUSION: Both sCD163 and mCD163 positive monocytes may have an active role in leprosy pathogenesis. They could be potential biomarkers for leprosy detection and typing.
Authors: H J Møller; H Aerts; H Grønbaek; N A Peterslund; P Hyltoft Petersen; N Hornung; L Rejnmark; E Jabbarpour; S K Moestrup Journal: Scand J Clin Lab Invest Suppl Date: 2002
Authors: Ricardo Luís Louzada Silva; Marcio B Santos; Priscila L S Almeida; Thayse S Barros; Lucas Magalhães; Rodrigo A Cazzaniga; Patrícia R M Souza; Nívea F Luz; Jaqueline França-Costa; Valeria M Borges; Djalma S Lima-Junior; Michael W Lipscomb; Malcolm S Duthie; Steven G Reed; Roque Pacheco Almeida; Amélia Ribeiro Jesus Journal: PLoS Negl Trop Dis Date: 2017-03-29