| Literature DB >> 3257931 |
M Ogawa1, T Mori, Y Mori, S Ueda, H Yoshida, I Kato, K Iesato, Y Wakashin, M Wakashin, K Okuda.
Abstract
The effects of prostaglandin E1 on cell-mediated cytotoxicity against hepatocytes were investigated using an in vitro cytotoxic assay system. Isolated liver cells from normal C57BL/6 mice were used as the target cells, and effector cells were obtained from spleens of C57BL/6 mice in which experimental hepatitis had been induced by immunization with syngeneic liver antigens. In this assay system, spleen T cells adhering to nylon wool demonstrated a high cytotoxic activity against target liver cells. The cytotoxicity was markedly reduced by prostaglandin E1 at concentrations greater than 10(-7) M. Maximum suppressive activity was obtained when prostaglandin E1 was continuously present during the assay period. By contrast, indomethacin, a specific inhibitor of prostaglandin synthesis, enhanced the cytotoxic activity of effector cells. These data seem to indicate that exogenously added prostaglandin E1 has an inhibitory effect on cell-mediated cytotoxicity of effector spleen cells against target hepatocytes.Entities:
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Year: 1988 PMID： 3257931 DOI： 10.1016/0016-5085(88)90562-8
Source DB: PubMed Journal: Gastroenterology ISSN： 0016-5085 Impact factor: 22.682