Monodisperse restricted access material with molecularly imprinted surface for selective solid-phase extraction of 17β-estradiol from milk.

In this study, a novel monodisperse restricted access media-molecularly imprinted polymers were successfully prepared via surface initiated reversible addition-fragmentation chain transfer polymerization using monodisperse crosslinked poly (glycidyl methacrylate-co-ethylene glycol dimethacrylate) microspheres as the carrier and acryloyl chloride-modified β-cyclodextrin as the hydrophilic functional monomer. The surface morphology, protein exclusion and adsorption properties of the molecularly imprinted polymers were investigated. The results show that the material has excellent monodispersity and hydrophilicity, and simultaneously exhibits high adsorption capacity, fast binding kinetics, high selectivity and significant thermal stability. The molecularly imprinted polymers as dispersive solid phase extraction adsorbent combined with reversed-phase HPLC were used to selectively enrich, separate and analyse trace 17β-estradiol in milk samples. The recovery of 17β-estradiol is 88-95% with relative standard deviation of less than 4%, and the limits of detection and quantification of this method are 2.08 and 9.29 μg·L-1 , respectively. The novel restricted access media-molecularly imprinted polymers adsorbent provide an effective method for the selective extraction and detection of 17β-estradiol directly from complex samples. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.