Xue-Hui Sun1, Xin Fan2, Kai-Li Hu3, Wen-Ting Hu1. 1. Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Weifang Medical University, Weifang 261000, China. 2. Dept. of Stomatology, The Affiliated Hospital of Weifang Medical University, Weifang 261000, China. 3. School of Stomatology, Weifang Medical University, Weifang 261021, China.
Abstract
OBJECTIVE: This study aimed to investigate the molecular mechanism of RAB1A in the proliferation, invasion, and metastasis of human tongue squamous cell carcinoma. METHODS: Western blot was used to detect the expression of RAB1A protein in human normal tongue epithelial cells (Hacat) and tongue squamous cell carcinoma Tca8113. The changes in RAB1A after plasmid transfection were also studied. The Tca8113 cells were named SiRAB1A/Tca8113 after RAB1A plasmid transfection. The expression of the epithelial-mesenchymal transition (EMT)-related markers of SiRAB1A/Tca8113 cells was also detected. CCK-8 assay was used to detect the proliferation of SiRAB1A/Tca8113 cells. Transwell and wound healing assays were used to detect the invasive and metastatic abilities of SiRAB1A/Tca8113 cells, respectively. RESULTS: Western blot results showed that the expression of RAB1A in tongue squamous cell carcinoma cells was significantly higher than that in Hacat. RAB1A decreased significantly after SiRAB1A plasmid transfection. CCK-8 proliferation assay showed that the proliferation of SiRAB1A/Tca8113 cells also decreased significantly. Transwell and wound healing assays demonstrated that the invasive and metastatic abilities of SiRAB1A/Tca8113 cells decreased significantly, respectively. In addition, Western blot results demonstrated that RAB1A deletion significantly increased the expression of E-cadherin and inhibited the expression of Vimentin. CONCLUSIONS: RAB1A could promote the proliferation, invasion, and metastasis of tongue squamous cell carcinoma cells.
OBJECTIVE: This study aimed to investigate the molecular mechanism of RAB1A in the proliferation, invasion, and metastasis of humantongue squamous cell carcinoma. METHODS: Western blot was used to detect the expression of RAB1A protein in human normal tongue epithelial cells (Hacat) and tongue squamous cell carcinoma Tca8113. The changes in RAB1A after plasmid transfection were also studied. The Tca8113 cells were named SiRAB1A/Tca8113 after RAB1A plasmid transfection. The expression of the epithelial-mesenchymal transition (EMT)-related markers of SiRAB1A/Tca8113 cells was also detected. CCK-8 assay was used to detect the proliferation of SiRAB1A/Tca8113 cells. Transwell and wound healing assays were used to detect the invasive and metastatic abilities of SiRAB1A/Tca8113 cells, respectively. RESULTS: Western blot results showed that the expression of RAB1A in tongue squamous cell carcinoma cells was significantly higher than that in Hacat. RAB1A decreased significantly after SiRAB1A plasmid transfection. CCK-8 proliferation assay showed that the proliferation of SiRAB1A/Tca8113 cells also decreased significantly. Transwell and wound healing assays demonstrated that the invasive and metastatic abilities of SiRAB1A/Tca8113 cells decreased significantly, respectively. In addition, Western blot results demonstrated that RAB1A deletion significantly increased the expression of E-cadherin and inhibited the expression of Vimentin. CONCLUSIONS:RAB1A could promote the proliferation, invasion, and metastasis of tongue squamous cell carcinoma cells.
Authors: Janice D Thomas; Yan-Jie Zhang; Yue-Hua Wei; Jun-Hung Cho; Laura E Morris; Hui-Yun Wang; X F Steven Zheng Journal: Cancer Cell Date: 2016-07-11 Impact factor: 31.743
Authors: Joseph E Tota; William F Anderson; Charles Coffey; Joseph Califano; Wendy Cozen; Robert L Ferris; Maie St John; Ezra E W Cohen; Anil K Chaturvedi Journal: Oral Oncol Date: 2017-02-28 Impact factor: 5.972