H-T Liu1, W-X Fan. 1. Department of Urology Surgery, Daqing Oilfield General Hospital, Daqing, China. fanwx1999@163.com.
Abstract
OBJECTIVE: To uncover the role of miRNA-1246 in influencing the proliferative and migratory capacities of RCC by binding CXCR4 and downregulating its level. PATIENTS AND METHODS: Relative levels of miRNA-1246 and CXCR4 in 40 paired RCC tissues and adjacent normal tissues were determined. The binding relationship between miRNA-1246 and CXCR4 was confirmed by Dual-Luciferase reporter assay. Proliferative and migratory abilities of RCC regulated by miRNA-1246 and CXCR4 were assessed. RESULTS: MiRNA-1246 was downregulated in RCC tissues and cell lines. Overexpression of miRNA-1246 attenuated proliferative and migratory capacities of 786-O and 769-P cells. CXCR4 was the direct target of miRNA-1246 and its level was negatively regulated by miRNA-1246. Silence of CXCR4 inhibited RCC to proliferate and migrate. CONCLUSIONS: MiRNA-1246 attenuates proliferative and migratory abilities of RCC by downregulating CXCR4. MiRNA-1246/CXCR4 axis could be potential therapeutic target for RCC.
OBJECTIVE: To uncover the role of miRNA-1246 in influencing the proliferative and migratory capacities of RCC by binding CXCR4 and downregulating its level. PATIENTS AND METHODS: Relative levels of miRNA-1246 and CXCR4 in 40 paired RCC tissues and adjacent normal tissues were determined. The binding relationship between miRNA-1246 and CXCR4 was confirmed by Dual-Luciferase reporter assay. Proliferative and migratory abilities of RCC regulated by miRNA-1246 and CXCR4 were assessed. RESULTS:MiRNA-1246 was downregulated in RCC tissues and cell lines. Overexpression of miRNA-1246 attenuated proliferative and migratory capacities of 786-O and 769-P cells. CXCR4 was the direct target of miRNA-1246 and its level was negatively regulated by miRNA-1246. Silence of CXCR4 inhibited RCC to proliferate and migrate. CONCLUSIONS:MiRNA-1246 attenuates proliferative and migratory abilities of RCC by downregulating CXCR4. MiRNA-1246/CXCR4 axis could be potential therapeutic target for RCC.
Authors: Lauretta Levati; Cristian Bassi; Simona Mastroeni; Laura Lupini; Gian Carlo Antonini Cappellini; Laura Bonmassar; Ester Alvino; Simona Caporali; Pedro Miguel Lacal; Maria Grazia Narducci; Ivan Molineris; Federica De Galitiis; Massimo Negrini; Giandomenico Russo; Stefania D'Atri Journal: Cancers (Basel) Date: 2022-07-29 Impact factor: 6.575