| Literature DB >> 32572232 |
Wenkai Yi1,2, Jingyu Li2,3, Xiaoxuan Zhu2,3, Xi Wang1,4, Ligang Fan1,2, Wenju Sun1, Linbu Liao1, Jilin Zhang5, Xiaoyu Li1,2, Jing Ye6, Fulin Chen1, Jussi Taipale5,7, Kui Ming Chan8,9, Liang Zhang10,11, Jian Yan12,13.
Abstract
We have developed CRISPR-assisted RNA-protein interaction detection method (CARPID), which leverages CRISPR-CasRx-based RNA targeting and proximity labeling to identify binding proteins of specific long non-coding RNAs (lncRNAs) in the native cellular context. We applied CARPID to the nuclear lncRNA XIST, and it captured a list of known interacting proteins and multiple previously uncharacterized binding proteins. We generalized CARPID to explore binders of the lncRNAs DANCR and MALAT1, revealing the method's wide applicability in identifying RNA-binding proteins.Entities:
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Year: 2020 PMID： 32572232 DOI： 10.1038/s41592-020-0866-0
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547