Marilena Marraudino1, Beatriz Carrillo2, Brigitta Bonaldo1, Ricardo Llorente3, Elia Campioli3, Iciar Garate4, Helena Pinos2, Luis Miguel Garcia-Segura5, Paloma Collado2, Daniela Grassi6,7,8. 1. Department of Neuroscience "Rita Levi Montalcini," Neuroscience Institute Cavalieri Ottolenghi, University of Turin, Turin, Italy. 2. Department of Psychobiology, Universidad Nacional de Educación a Distancia, Madrid, Spain. 3. Department of Preclinical Odontology, Universidad Europea de Madrid, Madrid, Spain. 4. Department of Physiotherapy, Podology, and Dance, Universidad Europea de Madrid, Madrid, Spain. 5. Instituto Cajal, CSIC, and Centro de Investigación Biomédica en Red Fragilidad y Envejecimiento Saludable, Instituto de Salud Carlos III, Madrid, Spain. 6. Department of Psychobiology, Universidad Nacional de Educación a Distancia, Madrid, Spain, daniela.grassi@universidadeuropea.es. 7. Department of Preclinical Odontology, Universidad Europea de Madrid, Madrid, Spain, daniela.grassi@universidadeuropea.es. 8. Instituto Cajal, CSIC, and Centro de Investigación Biomédica en Red Fragilidad y Envejecimiento Saludable, Instituto de Salud Carlos III, Madrid, Spain, daniela.grassi@universidadeuropea.es.
Abstract
INTRODUCTION: The membrane-associated G protein-coupled estrogen receptor 1 (GPER) mediates the regulation by estradiol of arginine-vasopressin immunoreactivity in the supraoptic and paraventricular hypothalamic nuclei of female rats and is involved in the estrogenic control of hypothalamic regulated functions, such as food intake, sexual receptivity, and lordosis behavior. OBJECTIVE: To assess GPER distribution in the rat hypothalamus. METHODS: GPER immunoreactivity was assessed in different anatomical subdivisions of five selected hypothalamic regions of young adult male and cycling female rats: the arcuate nucleus, the lateral hypothalamus, the paraventricular nucleus, the supraoptic nucleus, and the ventromedial hypothalamic nucleus. GPER immunoreactivity was colocalized with NeuN as a marker of mature neurons, GFAP as a marker of astrocytes, and CC1 as a marker of mature oligodendrocytes. RESULTS: GPER immunoreactivity was detected in hypothalamic neurons, astrocytes, and oligodendrocytes. Sex and regional differences and changes during the estrous cycle were detected in the total number of GPER-immunoreactive cells and in the proportion of neurons, astrocytes, and oligodendrocytes that were GPER-immunoreactive. CONCLUSIONS: These findings suggest that estrogenic regulation of hypothalamic function through GPER may be different in males and females and may fluctuate during the estrous cycle in females.
INTRODUCTION: The membrane-associated G protein-coupled estrogen receptor 1 (GPER) mediates the regulation by estradiol of arginine-vasopressin immunoreactivity in the supraoptic and paraventricular hypothalamic nuclei of female rats and is involved in the estrogenic control of hypothalamic regulated functions, such as food intake, sexual receptivity, and lordosis behavior. OBJECTIVE: To assess GPER distribution in the rat hypothalamus. METHODS: GPER immunoreactivity was assessed in different anatomical subdivisions of five selected hypothalamic regions of young adult male and cycling female rats: the arcuate nucleus, the lateral hypothalamus, the paraventricular nucleus, the supraoptic nucleus, and the ventromedial hypothalamic nucleus. GPER immunoreactivity was colocalized with NeuN as a marker of mature neurons, GFAP as a marker of astrocytes, and CC1 as a marker of mature oligodendrocytes. RESULTS: GPER immunoreactivity was detected in hypothalamic neurons, astrocytes, and oligodendrocytes. Sex and regional differences and changes during the estrous cycle were detected in the total number of GPER-immunoreactive cells and in the proportion of neurons, astrocytes, and oligodendrocytes that were GPER-immunoreactive. CONCLUSIONS: These findings suggest that estrogenic regulation of hypothalamic function through GPER may be different in males and females and may fluctuate during the estrous cycle in females.
Authors: Natalina H Contoreggi; Sanoara Mazid; Lily B Goldstein; John Park; Astrid C Ovalles; Elizabeth M Waters; Michael J Glass; Teresa A Milner Journal: J Comp Neurol Date: 2021-01-14 Impact factor: 3.028