INTRODUCTION: High rates of positive Cutibacterium acnes cultures from primary shoulder surgery make positive cultures in the revision surgery setting difficult to interpret. Our goal was to determine concordance between culture and next-generation sequencing techniques for identification of C. acnes from primary shoulder surgery. METHODS: Patients undergoing shoulder arthroscopy for rotator cuff tear or primary anatomic shoulder arthroplasty for osteoarthritis were prospectively enrolled. Specimens were sent for culture (aerobic and anaerobic) and next-generation sequencing. For next-generation sequencing, the entire microbial DNA was sequenced in order to identify pathogens within the sample. RESULTS: Our cohort consisted of 90 patients (45 patients in the osteoarthritis group and 45 patients in the rotator cuff tear group). At least one sample was positive for C. acnes in 18 (20%) cases by culture and in 14 (15.6%) cases by next-generation sequencing (p = 0.559). Four (22.2%) of the 18 cases with positive C. acnes cultures also had C. acnes identified by next-generation sequencing (kappa = 0.238). There was no difference in C. acnes identification rates between osteoarthritis and rotator cuff tear groups. CONCLUSIONS: There was limited concordance between culture and next-generation sequencing for C. acnes identification. Further studies are needed to determine the potential for next-generation sequencing as a diagnostic tool.
INTRODUCTION: High rates of positive Cutibacterium acnes cultures from primary shoulder surgery make positive cultures in the revision surgery setting difficult to interpret. Our goal was to determine concordance between culture and next-generation sequencing techniques for identification of C. acnes from primary shoulder surgery. METHODS: Patients undergoing shoulder arthroscopy for rotator cuff tear or primary anatomic shoulder arthroplasty for osteoarthritis were prospectively enrolled. Specimens were sent for culture (aerobic and anaerobic) and next-generation sequencing. For next-generation sequencing, the entire microbial DNA was sequenced in order to identify pathogens within the sample. RESULTS: Our cohort consisted of 90 patients (45 patients in the osteoarthritis group and 45 patients in the rotator cuff tear group). At least one sample was positive for C. acnes in 18 (20%) cases by culture and in 14 (15.6%) cases by next-generation sequencing (p = 0.559). Four (22.2%) of the 18 cases with positive C. acnes cultures also had C. acnes identified by next-generation sequencing (kappa = 0.238). There was no difference in C. acnes identification rates between osteoarthritis and rotator cuff tear groups. CONCLUSIONS: There was limited concordance between culture and next-generation sequencing for C. acnes identification. Further studies are needed to determine the potential for next-generation sequencing as a diagnostic tool.
Authors: Michael J Lee; Paul S Pottinger; Susan Butler-Wu; Roger E Bumgarner; Stacy M Russ; Frederick A Matsen Journal: J Bone Joint Surg Am Date: 2014-09-03 Impact factor: 5.284
Authors: Paul M Sethi; James R Sabetta; Samantha J Stuek; Storm V Horine; Katherine B Vadasdi; R Timothy Greene; James G Cunningham; Seth R Miller Journal: J Shoulder Elbow Surg Date: 2014-12-04 Impact factor: 3.019
Authors: Steven L Salzberg; Florian P Breitwieser; Anupama Kumar; Haiping Hao; Peter Burger; Fausto J Rodriguez; Michael Lim; Alfredo Quiñones-Hinojosa; Gary L Gallia; Jeffrey A Tornheim; Michael T Melia; Cynthia L Sears; Carlos A Pardo Journal: Neurol Neuroimmunol Neuroinflamm Date: 2016-06-13