Thiago Santos Rosa1, Rodrigo Vanerson Passos Neves2, Lysleine Alves Deus3, Caio Victor Sousa2, Samuel da Silva Aguiar2, Michel Kendy de Souza4, Milton Rocha Moraes2, Érica Carine Campos Caldas Rosa5, Rosangela Vieira Andrade6, Marko T Korhonen7, Herbert Gustavo Simões8. 1. Graduate Program in Physical Education, Catholic University of Brasília, Brasília, Brazil. Electronic address: thiagoacsdkp@yahoo.com.br. 2. Graduate Program in Physical Education, Catholic University of Brasília, Brasília, Brazil. 3. Graduate Program in Physical Education, Catholic University of Brasília, Brasília, Brazil. Electronic address: lys.deus@gmail.com. 4. Department of Nephrology, Federal University of São Paulo, São Paulo, Brazil. 5. Graduate Program in Health Sciences, University of Brasilia, Brasília, Brazil. 6. Program in Genomic Science and Biotechnology, Catholic University of Brasília, Brasília, Brazil. 7. Gerontology Research Center, Faculty of Sport and Health Sciences, University of Jyvaskyla, Finland. 8. Graduate Program in Physical Education, Catholic University of Brasília, Brasília, Brazil. Electronic address: hgsimoes@gmail.com.
Abstract
PURPOSE: Studies have shown a positive influence of intense athletic training on several biomarkers of aging, but it remains unclear whether this influence is dependent of exercise-training-mode. This study compared redox balance, cytokine levels and biomarkers of aging between master sprinters and endurance athletes, as well as in young and middle-aged individuals as controls. METHODS: Participants were male master sprinters (SA, 50 ± 8.9yrs; n = 13) and endurance runners (EA, 53 ± 8.2yrs; n = 18) with remarkable athletic experience (~25yrs of practice), besides untrained young (YC, 22.7 ± 3.9yrs; n = 17) and age-matched controls (MC, 45.5 ± 9.8yrs; n = 12). Anamnesis, anthropometrics, biomarkers of aging, inflammation status and oxidative stress parameters were analyzed in all participants. RESULTS: An increased pro-oxidant activity (elevated protein carbonyl; isoprostanes and 8-OHdG) was observed for MC in comparison to remaining groups (p < 0.05). However, SA presented a better antioxidant capacity than both MC and EA, while nitrite/nitrate (NOx) availability was higher for EA and lower for the MC (p < 0.05). Both groups of athletes presented a better anti-inflammatory status than MC (increased IL-10 and lowered IL-6, sIL-6R, sTNF-RI), but worse than YC (increased TNF-α, sTNF-RI, and sIL-6R) (p < 0.05). Telomere length was shorter in MC, which also had lower levels of irisin and klotho, and elevated FGF-23 (p < 0.05). ADMA levels were higher in MC and SA, while irisin was lower in EA when compared to SA and YC (p < 0.05). CONCLUSION: Master athletes presented better redox balance and inflammatory status, with decreased biomarkers of aging compared to control. Regarding exercise mode, a better NO- profile, as a marker of endothelial function, was observed for EA, whereas SA had a better redox balance, cytokines profile and attenuated biomarkers of aging.
PURPOSE: Studies have shown a positive influence of intense athletic training on several biomarkers of aging, but it remains unclear whether this influence is dependent of exercise-training-mode. This study compared redox balance, cytokine levels and biomarkers of aging between master sprinters and endurance athletes, as well as in young and middle-aged individuals as controls. METHODS:Participants were male master sprinters (SA, 50 ± 8.9yrs; n = 13) and endurance runners (EA, 53 ± 8.2yrs; n = 18) with remarkable athletic experience (~25yrs of practice), besides untrained young (YC, 22.7 ± 3.9yrs; n = 17) and age-matched controls (MC, 45.5 ± 9.8yrs; n = 12). Anamnesis, anthropometrics, biomarkers of aging, inflammation status and oxidative stress parameters were analyzed in all participants. RESULTS: An increased pro-oxidant activity (elevated protein carbonyl; isoprostanes and 8-OHdG) was observed for MC in comparison to remaining groups (p < 0.05). However, SA presented a better antioxidant capacity than both MC and EA, while nitrite/nitrate (NOx) availability was higher for EA and lower for the MC (p < 0.05). Both groups of athletes presented a better anti-inflammatory status than MC (increased IL-10 and lowered IL-6, sIL-6R, sTNF-RI), but worse than YC (increased TNF-α, sTNF-RI, and sIL-6R) (p < 0.05). Telomere length was shorter in MC, which also had lower levels of irisin and klotho, and elevated FGF-23 (p < 0.05). ADMA levels were higher in MC and SA, while irisin was lower in EA when compared to SA and YC (p < 0.05). CONCLUSION: Master athletes presented better redox balance and inflammatory status, with decreased biomarkers of aging compared to control. Regarding exercise mode, a better NO- profile, as a marker of endothelial function, was observed for EA, whereas SA had a better redox balance, cytokines profile and attenuated biomarkers of aging.
Authors: Sara Duarte Gutierrez; Samuel da Silva Aguiar; Lucas Pinheiro Barbosa; Patrick Anderson Santos; Larissa Alves Maciel; Patrício Lopes de Araújo Leite; Thiago Dos Santos Rosa; Lysleine Alves de Deus; John Eugene Lewis; Herbert Gustavo Simões Journal: J Clin Transl Res Date: 2021-07-16
Authors: Francis Ribeiro de Souza; Daisy Motta-Santos; Douglas Dos Santos Soares; Juliana Beust de Lima; Gustavo Gonçalves Cardozo; Luciano Santos Pinto Guimarães; Carlos Eduardo Negrão; Marcelo Rodrigues Dos Santos Journal: J Sci Med Sport Date: 2021-05-23 Impact factor: 4.319