Han Shuwen1, Yang Xi2, Pan Yuefen3, Xu Jiamin4, Qi Quan5, Liao Haihong6, Jiang Yizhen7, Wu Wei8. 1. Department of Oncology, Huzhou Cent Hosp, Affiliated Cent Hops HuZhou University. Address: 198 Hongqi Rd, Huzhou, Zhejiang, China. 2. Department of Intervention and Radiotherapy, Huzhou Central Hospital, Address: No. 198 Hongqi Road, Huzhou, Zhejiang Province, 313000, China. Electronic address: yangxi0601@hotmail.com. 3. Department of Oncology, Huzhou Cent Hosp, Affiliated Cent Hops HuZhou University. Address: 198 Hongqi Rd, Huzhou, Zhejiang, China. Electronic address: panyuefen663253@163.com. 4. Graduate School of Nursing, Huzhou University, Address: No. 1 Bachelor Road, Huzhou, Zhejiang Province, 313000, China. Electronic address: xjm996996@163.com. 5. Department of Oncology, Huzhou Cent Hosp, Affiliated Cent Hops HuZhou University. Address: 198 Hongqi Rd, Huzhou, Zhejiang, China. Electronic address: loveshohoo@163.com. 6. Department of Oncology, Huzhou Cent Hosp, Affiliated Cent Hops HuZhou University. Address: 198 Hongqi Rd, Huzhou, Zhejiang, China. Electronic address: haihonglll@126.com. 7. Department of Oncology, Huzhou Cent Hosp, Affiliated Cent Hops HuZhou University. Address: 198 Hongqi Rd, Huzhou, Zhejiang, China. Electronic address: jiangyizhen.1234@163.com. 8. Department of Gastroenterology, Huzhou Central Hospital, Address: No. 198 Hongqi Road, Huzhou, Zhejiang Province, 313000, China. Electronic address: ww@hzhospital.com.
Abstract
BACKGROUND: The gut microbiome changes are related to the colorectal cancer (CRC). Chemotherapy is one of the main treatment methods for CRC. PURPOSE: To explore the effect of chemotherapy on the gut bacteria and fungi in CRC. METHODS: Total of 11 advanced CRC patients treated with the FOLFIRI regimen, 15 postoperative CRC patients treated with the XELOX regimen, and corresponding CRC patients without surgery and chemotherapy were recruited. The 16S ribosomal RNA and ITS sequences were sequenced, and bioinformatics analysis was executed to screen for the distinctive gut microbiome. RESULTS: The abundances of Veillonella, Humicola, Tremellomycetes and Malassezia were increased in postoperative CRC patients treated with the XELOX regimen. The abundances of Faecalibacterium, Clostridiales, phascolarctobacterium, Humicola and Rhodotorula were decreased, and the abundances of Candida, Magnusiomyces, Tremellomycetes, Dipodascaceae, Saccharomycetales, Malassezia and Lentinula were increased in advanced CRC patients treated with the FOLFIRI regimen. The abundances of Humicola, Rhodotorula, and Magnusiomyces were decreased, and the abundances of Candida, Tremellomycetes, Dipodascaceae, Saccharomycetales, Malassezia and Lentinula were increased in advanced CRC patients treated with the FOLFIRI regimen combined with cetuximab compared with those treated with the FOLFIRI regimen alone. CONCLUSIONS: The community structure of gut bacteria and fungi changes in chemotherapy on CRCs.
BACKGROUND: The gut microbiome changes are related to the colorectal cancer (CRC). Chemotherapy is one of the main treatment methods for CRC. PURPOSE: To explore the effect of chemotherapy on the gut bacteria and fungi in CRC. METHODS: Total of 11 advanced CRCpatients treated with the FOLFIRI regimen, 15 postoperative CRCpatients treated with the XELOX regimen, and corresponding CRCpatients without surgery and chemotherapy were recruited. The 16S ribosomal RNA and ITS sequences were sequenced, and bioinformatics analysis was executed to screen for the distinctive gut microbiome. RESULTS: The abundances of Veillonella, Humicola, Tremellomycetes and Malassezia were increased in postoperative CRCpatients treated with the XELOX regimen. The abundances of Faecalibacterium, Clostridiales, phascolarctobacterium, Humicola and Rhodotorula were decreased, and the abundances of Candida, Magnusiomyces, Tremellomycetes, Dipodascaceae, Saccharomycetales, Malassezia and Lentinula were increased in advanced CRCpatients treated with the FOLFIRI regimen. The abundances of Humicola, Rhodotorula, and Magnusiomyces were decreased, and the abundances of Candida, Tremellomycetes, Dipodascaceae, Saccharomycetales, Malassezia and Lentinula were increased in advanced CRCpatients treated with the FOLFIRI regimen combined with cetuximab compared with those treated with the FOLFIRI regimen alone. CONCLUSIONS: The community structure of gut bacteria and fungi changes in chemotherapy on CRCs.