Rui Cao1, Min-Juan Zhang1, Yun-Tao Zhou2, Ya-Jie Liu1, Huan-Huan Wang1, Qin-Xin Zhang1, Ya-Wen Shi1, Jia-Chen Li3, Thian-Sze Wong4, Min Yin5. 1. Department of Otorhinolaryngology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. 2. Experimental Teaching Demonstration Centre, School of Rehabilitation Medicine, Nanjing Medical University, Nanjing, China. 3. Department of Otorhinolaryngology, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China. 4. Division of Head and Neck, the department of surgery, the faculty of medicine, The University of Hong Kong, Hong Kong, China. 5. Department of Otorhinolaryngology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. simisodo@126.com.
Abstract
PURPOSE: To study neurochemical reactions to chronic intermittent hypoxia (CIH) in the hypoglossal nucleus (HN) of rats. METHODS: Adult male Sprague-Dawley rats (n = 12) were randomly divided into two groups (the CIH and the control group). The CIH rats were housed in a hypoxic chamber with the fraction of oxygen volume alternating between 21% and 5% by providing air for 60 s and then providing nitrogen for 60 s from 8:30 am to 16:30 pm each day for 35 days. The control group was housed in a cabin with normal oxygen levels. We studied the expression of c-fos protein, 5-hydroxytryptamine (5-HT) positive terminals, and its 2A receptors in hypoglossal nuclei by immunohistochemistry. RESULTS: The expression of c-fos, 5-HT positive terminals, and accordingly 5-HT 2A receptors in the CIH group were significantly higher than that in the controls (p < 0.05). The ventral side of the HN showed a clearly higher expression of 5-HT and its 2A receptors than the dorsal side (p < 0.05). CONCLUSION: There were 2 responses of the HN to CIH. First, CIH induced a higher expression of 5-HT positive terminals and its 2A receptors, and second, this reaction was much more evident in ventral side than in the dorsal side. We postulate that these responses may serve to be a protective and compensatory mechanism for CIH.
PURPOSE: To study neurochemical reactions to chronic intermittent hypoxia (CIH) in the hypoglossal nucleus (HN) of rats. METHODS: Adult male Sprague-Dawley rats (n = 12) were randomly divided into two groups (the CIH and the control group). The CIHrats were housed in a hypoxic chamber with the fraction of oxygen volume alternating between 21% and 5% by providing air for 60 s and then providing nitrogen for 60 s from 8:30 am to 16:30 pm each day for 35 days. The control group was housed in a cabin with normal oxygen levels. We studied the expression of c-fos protein, 5-hydroxytryptamine (5-HT) positive terminals, and its 2A receptors in hypoglossal nuclei by immunohistochemistry. RESULTS: The expression of c-fos, 5-HT positive terminals, and accordingly 5-HT 2A receptors in the CIH group were significantly higher than that in the controls (p < 0.05). The ventral side of the HN showed a clearly higher expression of 5-HT and its 2A receptors than the dorsal side (p < 0.05). CONCLUSION: There were 2 responses of the HN to CIH. First, CIH induced a higher expression of 5-HT positive terminals and its 2A receptors, and second, this reaction was much more evident in ventral side than in the dorsal side. We postulate that these responses may serve to be a protective and compensatory mechanism for CIH.
Authors: Julian P Saboisky; Jane E Butler; David K McKenzie; Robert B Gorman; John A Trinder; David P White; Simon C Gandevia Journal: J Physiol Date: 2007-10-04 Impact factor: 5.182
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