Xiaoxue Huang1, Mingyue Zheng2,3, Yuling Yi1, Anamica Patel4, Zhen Song1, Yan Li5. 1. College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Sichuan, 611137, China. 2. School of Health and Rehabilitation, Chengdu University of Traditional Chinese Medicine, Sichuan, 611137, China. 3. Adelaide Medical School, University of Adelaide, Adelaide, SA, 5005, Australia. 4. Observatory Evidence Service, Public Health Wales, Cardiff, CF10 4BZ, UK. 5. College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Sichuan, 611137, China. liliana@cdutcm.edu.cn.
Abstract
OBJECTIVE: This study aimed to investigate the inhibitory effect of berberine hydrochloride (BH) on Candida albicans (C.albicans) ATCC10231 biofilm formation. RESULTS: This paper found a positive correlation between the concentration of BH and its inhibitory effect on the cellular activity of early biofilms because we found that 128 and 32 μg/mL BH significantly inhibited biofilm formation (P < 0.05). BH significantly inhibited the cellular activity in early biofilms, destroyed the microscopic morphology of C.albicans and reduced the thickness of the biofilm. Both 128 and 32 μg/mL concentration solutions of BH significantly inhibited biofilm formation (P < 0.05). We found that the inhibitory effect of BH solution was positively correlated with its concentration and 128 μg/mL BH was better than 4 μg/mL fluconazole. Additionally, the results of RT-PCR indicated that 128 and 32 μg/mL BH inhibited the expression of EFG1, HWP1, ECE1, and ALS1 (P < 0.05). CONCLUSION: The efficacy of BH in inhibiting the formation of C.albicans biofilm by killing the cells in the biofilm and destroying its structure; and the mechanism may be to down-regulate the expression of EFG1, HWP1, ECE1, and ALS1 in hyphae formation, thereby, retarding the morphological transformation of C. albicans.
OBJECTIVE: This study aimed to investigate the inhibitory effect of berberine hydrochloride (BH) on Candida albicans (C.albicans) ATCC10231 biofilm formation. RESULTS: This paper found a positive correlation between the concentration of BH and its inhibitory effect on the cellular activity of early biofilms because we found that 128 and 32 μg/mL BH significantly inhibited biofilm formation (P < 0.05). BH significantly inhibited the cellular activity in early biofilms, destroyed the microscopic morphology of C.albicans and reduced the thickness of the biofilm. Both 128 and 32 μg/mL concentration solutions of BH significantly inhibited biofilm formation (P < 0.05). We found that the inhibitory effect of BH solution was positively correlated with its concentration and 128 μg/mL BH was better than 4 μg/mL fluconazole. Additionally, the results of RT-PCR indicated that 128 and 32 μg/mL BH inhibited the expression of EFG1, HWP1, ECE1, and ALS1 (P < 0.05). CONCLUSION: The efficacy of BH in inhibiting the formation of C.albicans biofilm by killing the cells in the biofilm and destroying its structure; and the mechanism may be to down-regulate the expression of EFG1, HWP1, ECE1, and ALS1 in hyphae formation, thereby, retarding the morphological transformation of C. albicans.
Authors: Anderson Ramos da Silva; João Batista de Andrade Neto; Cecília Rocha da Silva; Rosana de Sousa Campos; Rose Anny Costa Silva; Daniel Domingues Freitas; Francisca Bruna Stefany Aires do Nascimento; Larissa Nara Dantas de Andrade; Letícia Serpa Sampaio; Thalles Barbosa Grangeiro; Hemerson Iury Ferreira Magalhães; Bruno Coêlho Cavalcanti; Manoel Odorico de Moraes; Hélio Vitoriano Nobre Júnior Journal: Antimicrob Agents Chemother Date: 2016-05-23 Impact factor: 5.191