Literature DB >> 32555789

An NMR based phosphodiesterase assay.

Madoka Akimoto1, Tianning Yu, Kody Moleschi, Katherine Van, Ganesh S Anand, Giuseppe Melacini.   

Abstract

We propose a phosphodiesterase assay based on 1D 1H NMR to monitor the hydrolysis of cyclic nucleotides directly, without requiring tags or the addition of exogenous reagents. The method is suitable to measure phosphodiesterase KM and kcat parameters and to identify phosphodiesterase inhibitors.

Entities:  

Year:  2020        PMID： 32555789     DOI： 10.1039/d0cc01673j

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  2 in total

1.  Sterically demanding macrocyclic Eu(iii) complexes for selective recognition of phosphate and real-time monitoring of enzymatically generated adenosine monophosphate.

Authors:  Samantha E Bodman; Colum Breen; Sam Kirkland; Simon Wheeler; Erin Robertson; Felix Plasser; Stephen J Butler
Journal:  Chem Sci       Date:  2022-02-11       Impact factor: 9.825

2.  Development of Phosphodiesterase-Protein-Kinase Complexes as Novel Targets for Discovery of Inhibitors with Enhanced Specificity.

Authors:  Nikhil K Tulsian; Valerie Jia-En Sin; Hwee-Ling Koh; Ganesh S Anand
Journal:  Int J Mol Sci       Date:  2021-05-15       Impact factor: 5.923
  2 in total

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