Literature DB >> 32553533

Stability of plasmid and viral banks supporting the cGMP manufacture of Q-Griffithsin from a TMV-based viral vector.

Julia M Corman1, Krystal T Hamorsky2, John W Shepherd3, Ernie Hiatt4, Joshua L Fuqua5, Kenneth E Palmer6.   

Abstract

The "whole genome" TMV-based expression system, Geneware®, was used in the cGMP production of the plant-made pharmaceutical Q-Griffithsin and demonstrates stable expression for up to a two-year period. Virion and plasmid banks which contained viral cDNA and a Q-Griffithsin sequence were able to produce >200 g of Q-Griffithsin. Data assessing the quality and stability of the product banks were measured through functional assessments of visual symptomology and product expression.
Copyright © 2020 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Microbicide; Plant-made pharmaceuticals; Q-Griffithsin; Stability; TMV; Virion

Year:  2020        PMID： 32553533      PMCID： PMC7397727          DOI： 10.1016/j.jbiotec.2020.06.009

Source DB:  PubMed          Journal:  J Biotechnol        ISSN： 0168-1656            Impact factor:   3.307


  9 in total

Review 1.  Production of pharmaceutical-grade recombinant aprotinin and a monoclonal antibody product using plant-based transient expression systems.

Authors:  Gregory P Pogue; Fakhrieh Vojdani; Kenneth E Palmer; Ernie Hiatt; Steve Hume; Jim Phelps; Lori Long; Natasha Bohorova; Do Kim; Michael Pauly; Jesus Velasco; Kevin Whaley; Larry Zeitlin; Stephen J Garger; Earl White; Yun Bai; Hugh Haydon; Barry Bratcher
Journal:  Plant Biotechnol J       Date:  2010-06       Impact factor: 9.803

2.  Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component.

Authors:  Barry R O'Keefe; Fakhrieh Vojdani; Viviana Buffa; Robin J Shattock; David C Montefiori; James Bakke; Jon Mirsalis; Anna-Lisa d'Andrea; Steven D Hume; Barry Bratcher; Carrie J Saucedo; James B McMahon; Gregory P Pogue; Kenneth E Palmer
Journal:  Proc Natl Acad Sci U S A       Date:  2009-03-30       Impact factor: 11.205

3.  Tobacco mosaic virus infection results in an increase in recombination frequency and resistance to viral, bacterial, and fungal pathogens in the progeny of infected tobacco plants.

Authors:  Palak Kathiria; Corinne Sidler; Andrey Golubov; Melanie Kalischuk; Lawrence M Kawchuk; Igor Kovalchuk
Journal:  Plant Physiol       Date:  2010-05-24       Impact factor: 8.340

4.  Heterologous sequences greatly affect foreign gene expression in tobacco mosaic virus-based vectors.

Authors:  S Shivprasad; G P Pogue; D J Lewandowski; J Hidalgo; J Donson; L K Grill; W O Dawson
Journal:  Virology       Date:  1999-03-15       Impact factor: 3.616

5.  Production of Recombinant Cholera Toxin B Subunit in Nicotiana benthamiana Using GENEWARE® Tobacco Mosaic Virus Vector.

Authors:  Lauren Moore; Krystal Hamorsky; Nobuyuki Matoba
Journal:  Methods Mol Biol       Date:  2016

6.  Aggregation of TMV CP plays a role in CP functions and in coat-protein-mediated resistance.

Authors:  S Asurmendi; R H Berg; T J Smith; M Bendahmane; R N Beachy
Journal:  Virology       Date:  2007-05-09       Impact factor: 3.616

Review 7.  Griffithsin: An Antiviral Lectin with Outstanding Therapeutic Potential.

Authors:  Sabrina Lusvarghi; Carole A Bewley
Journal:  Viruses       Date:  2016-10-24       Impact factor: 5.048

Review 8.  Bulk production of the antiviral lectin griffithsin.

Authors:  Joshua L Fuqua; Krystal Hamorsky; Guruatma Khalsa; Nobuyuki Matoba; Kenneth E Palmer
Journal:  Plant Biotechnol J       Date:  2015-07-14       Impact factor: 9.803

9.  Technoeconomic Modeling of Plant-Based Griffithsin Manufacturing.

Authors:  Aatif Alam; Linda Jiang; Gregory A Kittleson; Kenneth D Steadman; Somen Nandi; Joshua L Fuqua; Kenneth E Palmer; Daniel Tusé; Karen A McDonald
Journal:  Front Bioeng Biotechnol       Date:  2018-07-24
  9 in total
  2 in total

1.  Post-genomic platform for development of oligonucleotide vaccines against RNA viruses: diamond cuts diamond.

Authors:  V V Oberemok; O A Andreeva; K V Laikova; I A Novikov; A V Kubyshkin
Journal:  Inflamm Res       Date:  2022-05-06       Impact factor: 6.986

2.  In vitro Study on Synergistic Interactions Between Free and Encapsulated Q-Griffithsin and Antiretrovirals Against HIV-1 Infection.

Authors:  Farnaz Minooei; Joel R Fried; Joshua L Fuqua; Kenneth E Palmer; Jill M Steinbach-Rankins
Journal:  Int J Nanomedicine       Date:  2021-02-15
  2 in total

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