| Literature DB >> 32552756 |
Jing Zhang1, Xiao-Han Liu2, Cong Li2, Xiao-Xing Wu3, Yan-Lin Chen4, Wen-Wen Li5, Xian Li5, Fan Gong2, Qin Tang2, Dan Jiang2.
Abstract
An amendment to this paper has been published and can be accessed via the original article.Entities:
Year: 2020 PMID: 32552756 PMCID: PMC7301439 DOI: 10.1186/s13046-020-01596-w
Source DB: PubMed Journal: J Exp Clin Cancer Res ISSN: 0392-9078
Fig. 1SNCG is overexpressed in EOC tissue and correlated with poor prognosis in patients with HGSOC. a IHC staining showed SNCG expression in the normal ovary, fallopian tube tissues, benign tumor, and borderline tumor (original magnification, × 200). b Representative images of IHC staining of SNCG expression in different pathological types of EOC tissues (original magnification, upper× 200, lower× 400). c Kaplan-Meier analysis was performed for EOC patients to analyze the association between SNCG expression and survival outcome (OS and PFS). d In the HGSOC cohort, Kaplan-Meier analysis indicated the correlation of SNCG overexpression with OS (upper) and PFS (lower). By multivariate Cox regression analysis (Forest plot), results showed that SNCG overexpression was an independent prognostic factor
Fig. 2SNCG accelerates ovarian cancer cell proliferation, facilitates cell migration and invasion in vitro. a Western blot analysis of SNCG expression in different ovarian cancer cell lines. b The transfection efficiency was confirmed by Western blotting and qRT-PCR in SKOV3, HO-8901 PM, and OVCAR3 cells. c The MTT assay was used to detect ovarian cancer cell viability. d A soft agar assay was used to examine the proliferation of ovarian cancer cells. e Cell migration and invasion capabilities were determined using transwell assays (original magnification, × 200). f and g SKOV3 and HO-8910 PM cell transfectants were plated on FN and stained for SNCG, phalloidin, and nuclear. Moreover, cells were stained for Vimentin, MMP9, and F-actin. The individual or merged images visualized by a laser scanning confocal microscope (original magnification, × 1000). ▲, P < 0.05. *, P < 0.001. Ctrl: control; Ove: overexpression; Scr: scramble; sh1: small hairpin RNA 1; sh2: small hairpin RNA 2; sh3: small hairpin RNA 3