Literature DB >> 325517

Requirement of chain initiation factor 3 and ribosomal protein S1 in translation of synthetic and natural messenger RNA.

J E Sobura, M R Chowdhury, D A Hawley, A J Wahba.   

Abstract

Amino acid incorporation directed by poly(A), poly(U) or R17 RNA has been examined in S1-depleted protein synthesizing systems. We observe that the translation of either synthetic or natural messenger RNA is strictly dependent on the presence of chain initiation factor 3 and ribosomal protein S1. With poly(A) or poly(U) both IF-3 and S1 stimulate amino acid incorporation at least 25-fold, and with R17 RNA the stimulation is approximately 15-fold. More than one copy of S1 per ribosome decreases amino acid incorporation directed by poly(U) or R17 RNA. Initiation complex formation with R17 RNA is also stimulated optimally by the addition of one copy of S1 per ribosome. The function of IF-3 and S1 in protein synthesis is considered.

Entities:  

Mesh：

Substances：

Year:  1977        PMID： 325517      PMCID： PMC342406          DOI： 10.1093/nar/4.1.17

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  36 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Chain initiation factors from Escherichia coli.

Authors:  A J Wahba; M J Miller
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

3.  Polyuridylic acid binding and translating by Escherichia coli ribosomes: stimulation by protein I, inhibition by aurintricarboxylic acid.

Authors:  M Tal; M Aviram; A Kanarek; A Weiss
Journal:  Biochim Biophys Acta       Date:  1972-10-27

4.  The mechanism of action of initiation factor 3 in protein synthesis. II. Association of the 30S ribosomal protein S12 with IF-3.

Authors:  D A Hawley; L I Slobin; A J Wahba
Journal:  Biochem Biophys Res Commun       Date:  1974-11-27       Impact factor: 3.575

5.  Replacement of ribosomal protein S1 by interference factor ialpha in ribosomal binding of phage Ms2 RNA.

Authors:  J M Hermoso; W Szer
Journal:  Proc Natl Acad Sci U S A       Date:  1974-12       Impact factor: 11.205

6.  The mechanism of action of initiation factor 3 in protein synthesis. I. Studies on ribosomes crosslinked with dimethylsuberimidate.

Authors:  D A Hawley; M J Miller; L I Slobin; A J Wahba
Journal:  Biochem Biophys Res Commun       Date:  1974-11-06       Impact factor: 3.575

7.  Two forms of the 30 S ribosomal subunit of Escherichia coli.

Authors:  A E Dahlberg
Journal:  J Biol Chem       Date:  1974-12-10       Impact factor: 5.157

8.  Subunit I of G beta replicase and 30 S ribosomal protein S1 of Escherichia coli. Evidence for the identity of the two proteins.

Authors:  A J Wahba; M J Miller; A Niveleau; T A Landers; G G Carmichael; K Weber; D A Hawley; L I Slobin
Journal:  J Biol Chem       Date:  1974-05-25       Impact factor: 5.157

9.  Physical and functional homology between ribosomal protein S1 and interference factor i.

Authors:  H Inouye; Y Pollack; J Petre
Journal:  Eur J Biochem       Date:  1974-06-01

10.  Interaction of Escherichia coli 30S ribosomal subunits with MS2 phage RNA in the absence of initiation factors.

Authors:  W Szer; S Leffler
Journal:  Proc Natl Acad Sci U S A       Date:  1974-09       Impact factor: 11.205
View more
  9 in total

1.  The 3' terminus of 16S rRNA: secondary structure and interaction with ribosomal protein S1.

Authors:  R C Yuan; J A Steitz; P B Moore; D M Crothers
Journal:  Nucleic Acids Res       Date:  1979-12-20       Impact factor: 16.971

2.  Chain initiation factor 3 crosslinks to E. coli 30S and 50S ribosomal subunits and alters the UV absorbance spectrum of 70S ribosomes.

Authors:  J B Chaires; D A Hawley; A J Wahba
Journal:  Nucleic Acids Res       Date:  1982-09-25       Impact factor: 16.971

3.  Effects of growth conditions and mutations in RNA polymerase on translational activity in vitro in Escherichia coli.

Authors:  L A Isaksson; K Fodor; L A Kirsebom; F Bouadloun; E Murén
Journal:  Mol Gen Genet       Date:  1980

4.  Kinetic studies of the rates and mechanism of assembly of the protein synthesis initiation complex.

Authors:  D J Goss; L J Parkhurst; A J Wahba
Journal:  Biophys J       Date:  1980-10       Impact factor: 4.033

5.  Segmental flexibility in Escherichia coli ribosomal protein S1 as studied by fluorescence polarization.

Authors:  Y G Chu; C R Cantor
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971

6.  Localization of polyamine enhancement of protein synthesis to subcellular components of Escherichia coli and Pseudomonas sp. strain Kim.

Authors:  C L Rosano; S C Bunce; C Hurwitz
Journal:  J Bacteriol       Date:  1983-01       Impact factor: 3.490

7.  Mechanism of the interaction between ribosomal protein S1 and oligonucleotides.

Authors:  A Mülsch; M Colpan; E Wollny; H G Gassen; D Riesner
Journal:  Nucleic Acids Res       Date:  1981-05-25       Impact factor: 16.971

8.  Nuclease mapping of the secondary structure of the 49-nucleotide 3' terminal cloacin fragment of Escherichia coli 16s RNA and its interactions with initiation factor 3.

Authors:  E Wickstrom
Journal:  Nucleic Acids Res       Date:  1983-04-11       Impact factor: 16.971

9.  The binding of ribosomal protein S1 to S1-depleted 30S and 70S ribosomes. A fluorescence anisotropy study of the effects of Mg2+.

Authors:  D J Goss; L J Parkhurst; A M Mehta; A J Wahba
Journal:  Nucleic Acids Res       Date:  1983-08-25       Impact factor: 16.971
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.