| Literature DB >> 32544240 |
Naoki Uno1, Norihito Kaku1, Yoshitomo Morinaga1, Hiroo Hasegawa1, Katsunori Yanagihara1.
Abstract
Specific nucleic acid sequences can be detected in individual cells by in situ hybridization. However, when very few copies of a target sequence are present per cell, its signal is undetectable by flow cytometry. Although various approaches have been developed to increase fluorescence signals for in situ hybridization, flow cytometric detection of specific genomic DNA sequences has not been established. Here, we present a flow cytometry assay for detection of single-copy genomic sequences in human lymphocytes using in situ PCR with universal energy transfer-labelled primers.Entities:
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Year: 2020 PMID： 32544240 PMCID： PMC7470942 DOI： 10.1093/nar/gkaa515
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971