B Veyrand-Quirós1, B Gómez-Gil2, C O Lomeli-Ortega1, C Escobedo-Fregoso3, A D Millard4, D Tovar-Ramírez1, J L Balcázar5,6, E Quiroz-Guzmán3. 1. Centro de Investigaciones Biológicas del Noroeste, S.C., Av. Instituto Politécnico Nacional 195, La Paz Baja California Sur, Mexico. 2. Centro de Investigación en Alimentación y Desarrollo (CIAD), Unidad Mazatlán en Acuicultura y Manejo Ambiental, Mazatlán, Sinaloa, Mexico. 3. CONACYT-CIBNOR, Av. Instituto Politécnico Nacional, La Paz Baja California Sur, Mexico. 4. Department of Genetics and Genome Biology, University of Leicester, Leicester, UK. 5. Catalan Institute for Water Research (ICRA), Girona, Spain. 6. University of Girona, Girona, Spain.
Abstract
AIMS: This study describes the effect of phage therapy on hatching of longfin yellowtail (Seriola rivoliana) eggs challenged with Photobacterium damselae subsp. damselae. METHODS AND RESULTS: A lytic phage (vB_Pd_PDCC-1) against P. damselae subsp. damselae was isolated and characterized. The use of phage vB_Pd_PDCC-1 increased the hatching rate of eggs, and reduced presumptive Vibrio species to non-detectable numbers, even in non-disinfected eggs. High-throughput 16S rRNA gene sequencing analysis revealed that phage vB_Pd_PDCC-1 caused significant changes in the composition and structure of the associated microbiota, allowing that members (e.g. those belonging to the family Vibrionaceae) of the class Gammaproteobacteria to be displaced by members of the class Alphaproteobacteria. CONCLUSIONS: To the best of our knowledge, this represents the first study evaluating phage therapy to control potential negative effects of P. damselae subsp. damselae during hatching of longfin yellowtail eggs. SIGNIFICANCE AND IMPACT OF THE STUDY: The Seriola genus includes several important commercial fish species due to its rapid growth and easy adaptability to confinement conditions. However, bacterial infections (especially those caused by Vibrio and Photobacterium species) are among the main limiting factors for the intensification of marine fish aquaculture, particularly during early development stages. Therefore, the use of phages, which are natural killers of bacteria, represents a promising strategy to reduce the mortality of farmed organisms caused by pathogenic bacteria.
AIMS: This study describes the effect of phage therapy on hatching of longfin yellowtail (Seriola rivoliana) eggs challenged with Photobacterium damselae subsp. damselae. METHODS AND RESULTS: A lytic phage (vB_Pd_PDCC-1) against P. damselae subsp. damselae was isolated and characterized. The use of phage vB_Pd_PDCC-1 increased the hatching rate of eggs, and reduced presumptive Vibrio species to non-detectable numbers, even in non-disinfected eggs. High-throughput 16S rRNA gene sequencing analysis revealed that phage vB_Pd_PDCC-1 caused significant changes in the composition and structure of the associated microbiota, allowing that members (e.g. those belonging to the family Vibrionaceae) of the class Gammaproteobacteria to be displaced by members of the class Alphaproteobacteria. CONCLUSIONS: To the best of our knowledge, this represents the first study evaluating phage therapy to control potential negative effects of P. damselae subsp. damselae during hatching of longfin yellowtail eggs. SIGNIFICANCE AND IMPACT OF THE STUDY: The Seriola genus includes several important commercial fish species due to its rapid growth and easy adaptability to confinement conditions. However, bacterial infections (especially those caused by Vibrio and Photobacterium species) are among the main limiting factors for the intensification of marine fish aquaculture, particularly during early development stages. Therefore, the use of phages, which are natural killers of bacteria, represents a promising strategy to reduce the mortality of farmed organisms caused by pathogenic bacteria.
Authors: Bernardo Veyrand-Quirós; Laura T Guzmán-Villanueva; Ana G Reyes; Carmen Rodríguez-Jaramillo; Joan S Salas-Leiva; Dariel Tovar-Ramírez; José L Balcázar; Eduardo Quiroz-Guzman Journal: Appl Microbiol Biotechnol Date: 2021-03-12 Impact factor: 4.813