Literature DB >> 3253295

Fracture-flip: new high-resolution images of cell surfaces after carbon stabilization of freeze-fractured membranes.

C Andersson Forsman1, P Pinto da Silva.   

Abstract

We introduce fracture-flip, a method of obtaining high-resolution views of membrane surfaces. The method, a corollary of label-fracture, is based on the stabilization of the exoplasmic halves of membranes by carbon evaporation. Inversion of these casts followed by Pt/C imaging leads to new views of the cell surface at macromolecular resolution.

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Year:  1988        PMID： 3253295     DOI： 10.1242/jcs.90.4.531

Source DB:  PubMed          Journal:  J Cell Sci        ISSN： 0021-9533            Impact factor:   5.285


  5 in total

Review 1.  Freeze-fracture immunogold labeling.

Authors:  M R Torrisi; P Mancini
Journal:  Histochem Cell Biol       Date:  1996-07       Impact factor: 4.304

2.  Direct visualization of the interrelationship between intramembrane and extracellular structures.

Authors:  J B Wade; R A Coleman
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

3.  Giardia duodenalis: a freeze-fracture, fracture-flip and cytochemistry study.

Authors:  W M Kattenbach; P F Pimenta; W de Souza; P Pinto da Silva
Journal:  Parasitol Res       Date:  1991       Impact factor: 2.289

4.  Patching and capping of LFA-1 molecules on human lymphocytes.

Authors:  A Pavan; G Lucania; T Sansolini; L Frati; M R Torrisi
Journal:  Histochemistry       Date:  1992-11

5.  Improved antigen retrieval in freeze-fracture cytochemistry by evaporation of carbon as first replication layer.

Authors:  Wiebke Schlörmann; Markus John; Frank Steiniger; Martin Westermann; Walter Richter
Journal:  Histochem Cell Biol       Date:  2007-04-06       Impact factor: 2.531
  5 in total

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