Alemeh Mohammadzadeh1, Behzad Baradaran2, Narges Dastmalchi1, Shahryar Hashemzadeh3, Reza Safaralizadeh4. 1. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran. 2. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. 3. Liver and Gastrointestinal Disease Research Center and Department of General and Thoracic Surgery, Tabriz University of Medical Sciences, Tabriz, Iran. 4. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran. safaralizadeh@tabrizu.ac.ir.
Abstract
PURPOSE: The role of Lnc_OC1 in gastric cancer (GC) has not been well documented. The very purpose of the present study was to determine not only the expression of Lnc_OC1 in gastric tissues but also its role in the formation of GC. Furthermore, the expression levels of Lnc-OC1 were examined in H. pylori-positive versus H. pylori-negative GC tissues. METHODS: Tumor and adjacent normal tissues were collected from 43 patients with GC. RNA extraction and cDNA synthesis were performed. Then, qRT-PCR was carried out. Finally, an independent sample t test was run to examine the expression level of Lnc_OC1 in a GC and normal tissues using SPSS program. RESULTS: The results revealed a significantly higher expression level of Lnc_OC1 in the GC tissues as compared with the normal tissues (p = 0.0037). The correlations between the expression level of Lnc_OC1 and the clinical features of patients were not statistically significant (p > 0.05). Moreover, the expression of Lnc-OC1 was significantly higher in H. pylori-positive patients as compared with H. pylori-negative patients (p = 0.01). CONCLUSION: The findings of the present study revealed that deregulation of Lnc_OC1 may have a role in the H. pylori-associated pathogenesis of GC. Moreover, a direct association can be speculated between GC formation and Lnc_OC1 expression. The mentioned findings highlight the potential role of Lnc_OC1 as a prognostic biomarker of GC. Hence, further evaluations are required in this respect.
PURPOSE: The role of Lnc_OC1 in gastric cancer (GC) has not been well documented. The very purpose of the present study was to determine not only the expression of Lnc_OC1 in gastric tissues but also its role in the formation of GC. Furthermore, the expression levels of Lnc-OC1 were examined in H. pylori-positive versus H. pylori-negative GC tissues. METHODS: Tumor and adjacent normal tissues were collected from 43 patients with GC. RNA extraction and cDNA synthesis were performed. Then, qRT-PCR was carried out. Finally, an independent sample t test was run to examine the expression level of Lnc_OC1 in a GC and normal tissues using SPSS program. RESULTS: The results revealed a significantly higher expression level of Lnc_OC1 in the GC tissues as compared with the normal tissues (p = 0.0037). The correlations between the expression level of Lnc_OC1 and the clinical features of patients were not statistically significant (p > 0.05). Moreover, the expression of Lnc-OC1 was significantly higher in H. pylori-positive patients as compared with H. pylori-negative patients (p = 0.01). CONCLUSION: The findings of the present study revealed that deregulation of Lnc_OC1 may have a role in the H. pylori-associated pathogenesis of GC. Moreover, a direct association can be speculated between GC formation and Lnc_OC1 expression. The mentioned findings highlight the potential role of Lnc_OC1 as a prognostic biomarker of GC. Hence, further evaluations are required in this respect.