| Literature DB >> 32529373 |
Abstract
Dynamin-like protein 1 (Drp1) is the master regulator of mitochondrial fission. Drp1 translocates from the cytosol to the mitochondrial outer membrane to execute the scission process. Here we describe an immunofluorescence-based method to measure the mitochondrial translocation of Drp1 and quantify Drp1-related mitochondrial fission by labeling the mitochondrial import receptor subunit TOM20 in fixed cell culture.Entities:
Keywords: Confocal microscopy; Dynamin-related protein 1; Immunofluorescence staining; Mitochondrial fission; TOM20
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Year: 2020 PMID： 32529373 PMCID： PMC8106401 DOI： 10.1007/978-1-0716-0676-6_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745