Yang-Yang Jiang1, Bin Xu, Bing Fang. 1. Department of Orthodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology. Shanghai 200011, China. E-mail:daisyjyy_9th@163.com.
Abstract
PURPOSE: To investigate the characteristic expression of apoptotic genes in epulis. METHODS: Thirty-six patients with epulis were recruited in this study, and related tumor and normal gingival tissues were collected. Six pairs of samples were used for PCR array analysis of apoptosis, and the other thirty pairs of samples were used for qRT-PCR replication. The expression levels of related genes were calculated with internal reference gene delta ΔΔCt. The data were analyzed with SPSS 17.0 software package. RESULTS: Compared with the normal gingival tissues, the expression levels of AIFM1, BCL2, BCL2L1, BCL2L2, BFAR, BIRC2, BIRC3, BIRC6, BNIP2, BNIP3, CD40LG, and XIAP were significantly increased in the gingival tissues, while the expression level of TNFRSF25 was decreased. CONCLUSIONS: Over-expression of anti-apoptotic genes in Bcl-2 family and IAP family inhibits apoptosis of gingival tissues, which eventually causes epulis.
PURPOSE: To investigate the characteristic expression of apoptotic genes in epulis. METHODS: Thirty-six patients with epulis were recruited in this study, and related tumor and normal gingival tissues were collected. Six pairs of samples were used for PCR array analysis of apoptosis, and the other thirty pairs of samples were used for qRT-PCR replication. The expression levels of related genes were calculated with internal reference gene delta ΔΔCt. The data were analyzed with SPSS 17.0 software package. RESULTS: Compared with the normal gingival tissues, the expression levels of AIFM1, BCL2, BCL2L1, BCL2L2, BFAR, BIRC2, BIRC3, BIRC6, BNIP2, BNIP3, CD40LG, and XIAP were significantly increased in the gingival tissues, while the expression level of TNFRSF25 was decreased. CONCLUSIONS: Over-expression of anti-apoptotic genes in Bcl-2 family and IAP family inhibits apoptosis of gingival tissues, which eventually causes epulis.