| Literature DB >> 32520527 |
Yamin Li1, Raissa Li1, Anirban Chakraborty1, Roza Ogurlu1, Xuewei Zhao1, Jinjin Chen1, Qiaobing Xu1.
Abstract
Lipidoid nanoparticles have been demonstrated to be effective for intracellular delivery of small molecule drugs, proteins, and nucleic acids. Stimuli-responsive lipidoid nanoparticles are able to further improve delivery efficacy and reduce carrier-induced toxicity. Our group previously developed reduction and photoresponsive combinatorial libraries of lipidoid nanoparticles for small molecule and biologics delivery. Herein, we describe the synthesis, characterization, and intracellular mRNA delivery application of a new library of pH-responsive lipidoid nanoparticles. The acid-degradable cyclic benzylidene acetal-containing cationic lipidoids (R-O16CBA) were synthesized through a multistep reaction and characterized by NMR and MS. The acid-triggered degradation of lipidoids was studied using NMR, MS, DLS, and TEM. The results revealed that the R-O16CBA lipidoid can be completely degraded at pH 5. The R-O16CBA lipidoid nanoparticles were then fabricated with different formulations of DOPE and cholesterol and tested in vitro for intracellular mRNA delivery.Entities:
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Year: 2020 PMID： 32520527 PMCID： PMC9069324 DOI： 10.1021/acs.bioconjchem.0c00295
Source DB: PubMed Journal: Bioconjug Chem ISSN： 1043-1802 Impact factor: 6.069