Johnathan M Sheele1, Brian Ferrari2, Jerome Goddard3, Danie Schlatzer4, Kathleen C Lundberg4, Katirina Guinto5, Monica E Embers6, Andrew B Young7, Gale E Ridge8, Giovanni Damiani7, Thomas S McCormick7. 1. Department of Emergency Medicine, Mayo Clinic, Jacksonville, Florida, USA. 2. Immune Function Core Facility, Case Western Reserve University, Cleveland, Ohio, USA. 3. Department of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, Starkville, Mississippi, USA. 4. Center for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, Ohio, USA. 5. Case Western Reserve University, Cleveland, Ohio, USA. 6. Division of Immunology, Tulane University National Primate Research Center, Covington, Louisiana, USA. 7. Department of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA. 8. The Connecticut Agricultural Experiment Station, New Haven, Connecticut, USA.
Abstract
AIMS: To investigate the immunoglobulin (Ig) G response after being fed upon by Cimex lectularius L. METHODS AND RESULTS: Participants were fed upon by three male C lectularius insects weekly for a month. Blood was obtained before the feeding and at the last feeding, which was used for immunoblots against bed bug salivary gland extract, with antihuman Immunoglobulin G (IgG) secondary antibodies. No consistent IgG changes developed in 11 humans serially fed upon by C lectularius. Two participants had new IgG responses to proteins at molecular weights of approximately 12-13 kDa, and one had an IgG response to a protein at approximately 40 kDa. At the last study visit, more intense IgG bands to proteins at molecular weights of 12-13 kDa had developed in 55% of participants (6/11) and at molecular weights of ≈30, ≈40 and ≈70 kDa in 45% (5/11) compared with the first study visit. Nitrophorin and apyrase were the most common C lectularius proteins identified with liquid chromatography-tandem mass spectrometry in both crushed bed bug salivary gland extract and post-bed bug feeding extract. CONCLUSIONS: Human participants did not have consistent IgG responses to crushed C lectularius salivary gland extract.
AIMS: To investigate the immunoglobulin (Ig) G response after being fed upon by Cimex lectularius L. METHODS AND RESULTS:Participants were fed upon by three male C lectularius insects weekly for a month. Blood was obtained before the feeding and at the last feeding, which was used for immunoblots against bed bug salivary gland extract, with antihuman Immunoglobulin G (IgG) secondary antibodies. No consistent IgG changes developed in 11 humans serially fed upon by C lectularius. Two participants had new IgG responses to proteins at molecular weights of approximately 12-13 kDa, and one had an IgG response to a protein at approximately 40 kDa. At the last study visit, more intense IgG bands to proteins at molecular weights of 12-13 kDa had developed in 55% of participants (6/11) and at molecular weights of ≈30, ≈40 and ≈70 kDa in 45% (5/11) compared with the first study visit. Nitrophorin and apyrase were the most common C lectularius proteins identified with liquid chromatography-tandem mass spectrometry in both crushed bed bug salivary gland extract and post-bed bug feeding extract. CONCLUSIONS:Humanparticipants did not have consistent IgG responses to crushed C lectularius salivary gland extract.
Authors: A Cuéllar; A Rodríguez; E Halpert; F Rojas; A Gómez; A Rojas; E García Journal: Allergol Immunopathol (Madr) Date: 2010-03-12 Impact factor: 1.667
Authors: Daniela Schlatzer; Aiman A Haqqani; Xiaolin Li; Curtis Dobrowolski; Mark R Chance; John C Tilton Journal: Anal Chem Date: 2017-05-03 Impact factor: 6.986