Hepatoprotective effect of limonene against chronic immobilization induced liver damage in rats.

Prolonged immobilization may impair the physiological functions of various organs of the body, including the liver, brain, and heart. In this study, we investigated the hepatoprotective effect of limonene (a monoterpene) in male rats exposed to chronic immobilization. Rats were exposed to immobilization stress (6 h/21 days) and received limonene (10 mg/kg, oral gavage) during this period. Chronic immobilization increased the levels of liver enzymes alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase in serum. Increased levels of malondialdehyde and decreased glutathione content were also observed in the liver tissue of immobilized rats. Expression of TNF-α, IL-1β, IL-6, and NF-κB mRNA was increased, and infiltrated cells were also observed in the liver parenchyma in rats exposed to chronic immobilization. Limonene prevented all these changes in immobilized rats. These results suggest that limonene, due to its antioxidant and anti-inflammatory effects, rescues the liver from damages caused by chronic immobilization.