Effects of dissolved oxygen concentrations on a bioaugmented sequencing batch rector treating aniline-laden wastewater: Reactor performance, microbial dynamics and functional genes.

This work compared the efficiencies and internal reasons for aniline removal in a bioaugmented sequencing batch reactor at elevated dissolved oxygen (DO) concentrations. Nearly complete aniline removal was achieved while medium DO of 2.65 mg/L was optimal for subsequent nitrogen removal via heterotrophic nitrification-aerobic denitrification. Apart from the highest bacterial diversity richness, favorable DO condition largely enriched putatively aniline degrader, nitrifiers and denitrifiers. Further evidence from qPCR confirmed that moderate DO significantly stimulated the functional genes including aniline degrading gene tad, nitrifying genes amoA, hao, and denitrifying genes nirS and napA, respectively. This study indicates that the significant enrichment of key microorganisms and effective functional genes under optimal DO is the inner mechanism for reliable aniline degradation and subsequent nitrogen removal in the activated sludge reactor.