Sanne Kjær Hansen1, Hülya Kaya2, Louise Roer2, Frank Hansen2, Sissel Skovgaard2, Ulrik Stenz Justesen3, Dennis Schrøder Hansen4, Leif Percival Andersen5, Jenny Dahl Knudsen6, Bent L Røder7, Claus Østergaard8, Turid Søndergaard9, Esad Dzajic10, Mikala Wang11, Jurgita Samulioniené12, Henrik Hasman2, Anette M Hammerum13. 1. Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen S, Denmark; Department of Clinical Microbiology, Odense University Hospital, Odense C, Denmark. 2. Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen S, Denmark. 3. Department of Clinical Microbiology, Odense University Hospital, Odense C, Denmark. 4. Department of Clinical Microbiology, Herlev and Gentofte Hospital, Herlev, Denmark. 5. Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark. 6. Department of Clinical Microbiology, Hvidovre University Hospital, Hvidovre, Denmark. 7. Department of Clinical Microbiology, Zealand University Hospital, Slagelse, Denmark. 8. Department of Clinical Microbiology, Lillebaelt Hospital, Vejle, Denmark. 9. Department of Clinical Microbiology, Hospital Sønderjylland, Sønderborg, Denmark. 10. Department of Clinical Microbiology, Hospital South West Jutland, Esbjerg, Denmark. 11. Department of Clinical Microbiology, Aarhus University Hospital, Aarhus, Denmark. 12. Department of Clinical Microbiology, Aalborg University Hospital, Aalborg, Denmark. 13. Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen S, Denmark. Electronic address: ama@ssi.dk.
Abstract
OBJECTIVES: The aim of the study was to molecularly characterize third-generation cephalosporin-resistant Klebsiella pneumoniae isolated from bloodstream infections in Denmark in 2018 using whole-genome sequencing (WGS) data, and to compare these isolates to the most common clones detected in 2006 and 2008. METHODS: Sixty-two extended-spectrum beta-lactamase (ESBL)/AmpC-producing K. pneumoniae isolates from Danish blood cultures from 2018 were analysed using WGS to obtain multilocus sequence typing (MLST), core genome MLST (cgMLST), resistance profile and phylogeny. These were compared to the most common ESBL K. pneumoniae clones detected in 2006 and 2008. RESULTS: The most common ESBL clone was ST15 CTX-M-15, the DHA-1 enzyme was the most common in AmpC isolates, and the OXA-48-like group was the most common carbapenemase. Thirty-nine different sequence types (STs) were found, with the most frequent being ST14, ST15 and ST37, accounting for 24% of the isolates. The isolates were subdivided into 55 complex types (CTs) of which 49 were singletons, with the most frequent being ST14-CT2080. Two of the CTX-M-15-producing isolates from 2018 belonged to the ST15-CT105/CT3078 clone, which was first detected in 2006. CONCLUSIONS: The ESBL/AmpC K. pneumoniae isolates detected in Danish blood cultures belonged to many different types. No dominant clones were circulating in Danish hospitals, but the ST15-CT105/CT3078 CTX-M-15 K. pneumoniae clone was seen 13 years after its first detection.
OBJECTIVES: The aim of the study was to molecularly characterize third-generation cephalosporin-resistant Klebsiella pneumoniae isolated from bloodstream infections in Denmark in 2018 using whole-genome sequencing (WGS) data, and to compare these isolates to the most common clones detected in 2006 and 2008. METHODS: Sixty-two extended-spectrum beta-lactamase (ESBL)/AmpC-producing K. pneumoniae isolates from Danish blood cultures from 2018 were analysed using WGS to obtain multilocus sequence typing (MLST), core genome MLST (cgMLST), resistance profile and phylogeny. These were compared to the most common ESBL K. pneumoniae clones detected in 2006 and 2008. RESULTS: The most common ESBL clone was ST15 CTX-M-15, the DHA-1 enzyme was the most common in AmpC isolates, and the OXA-48-like group was the most common carbapenemase. Thirty-nine different sequence types (STs) were found, with the most frequent being ST14, ST15 and ST37, accounting for 24% of the isolates. The isolates were subdivided into 55 complex types (CTs) of which 49 were singletons, with the most frequent being ST14-CT2080. Two of the CTX-M-15-producing isolates from 2018 belonged to the ST15-CT105/CT3078 clone, which was first detected in 2006. CONCLUSIONS: The ESBL/AmpCK. pneumoniae isolates detected in Danish blood cultures belonged to many different types. No dominant clones were circulating in Danish hospitals, but the ST15-CT105/CT3078 CTX-M-15K. pneumoniae clone was seen 13 years after its first detection.
Authors: Xiaoyan Su; Xia Yan; Yunli Li; Dongsheng Zhang; Lin Li; Yi Geng; Fei Su; Chanjuan Yue; Rong Hou; Songrui Liu Journal: BMC Vet Res Date: 2022-05-17 Impact factor: 2.792
Authors: Patrick Butaye; Marc Stegger; Arshnee Moodley; Peter Damborg; Andrea Williams; Iona Halliday-Simmonds; Luca Guardabassi Journal: Antibiotics (Basel) Date: 2021-12-30