Jiawei Ji1, Qian Liu2, Rui Wang3, Tingting Luo4, Xiaokui Guo3, Mengsha Xu3, Qian Yin3, Xuetong Wang3, Min Zhou5, Min Li6, Ping He7. 1. Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China; Department of Medical Microbiology and Immunology, School of Medicine, Shanghai Jiaotong University, Shanghai, China. 2. Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China. 3. Department of Medical Microbiology and Immunology, School of Medicine, Shanghai Jiaotong University, Shanghai, China. 4. Department of Pharmaceutical Engineering, Zhejiang Pharmaceutical College, Ningbo, China. 5. Institute of Respiratory Diseases, School of Medicine, Shanghai Jiaotong University, Shanghai, China. 6. Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China. Electronic address: ruth_limin@126.com. 7. Department of Medical Microbiology and Immunology, School of Medicine, Shanghai Jiaotong University, Shanghai, China. Electronic address: hpatsh@sjtu.edu.cn.
Abstract
INTRODUCTION: Staphylococcus aureus is a common human pathogen that causes various diseases including infections on the skin, in the bloodstream and the lower respiratory tracts. The emergence of methicillin-resistant S. aureus (MRSA) made the treatment of the bacterial infection more difficult, calling for development of new therapeutics. Compared with conventional antibiotic therapy, phage therapy offers a promising alternative to combat infections caused by MRSA. RESULTS: Here we showed that phage VB_SauS_SH-St 15644 isolated from sewage inhibited MRSA isolates in vitro and in the murine skin infection model. Phage VB_SauS_SH-St 15644 belongs to Siphoviridae. The genome of the phage is a linear, 45,111 bp double-stranded DNA with GC content of 33.35%. Among the 37 clinical MRSA isolates tested, 12 (32%) were lysed by the phage in vitro. The phage was relatively stable at temperatures up to 40 °C or between pH 6 and 9. However, the phage was sensitive to UV light. 80% of the phage was approximately adsorbed to the host MRSA isolate in 4 min. The one-step growth curve showed that the latent period was about 12 min followed by the growth period (about 9 min). The burst size was estimated at 13 PFU per infected cell. Furthermore, in a murine skin infection model, the phage significantly inhibited MRSA infection. CONCLUSIONS: Our study suggested that phage VB_SauS_SH-St 15644 has a potential to inhibit MRSA skin infection.
INTRODUCTION:Staphylococcus aureus is a common human pathogen that causes various diseases including infections on the skin, in the bloodstream and the lower respiratory tracts. The emergence of methicillin-resistant S. aureus (MRSA) made the treatment of the bacterial infection more difficult, calling for development of new therapeutics. Compared with conventional antibiotic therapy, phage therapy offers a promising alternative to combat infections caused by MRSA. RESULTS: Here we showed that phage VB_SauS_SH-St 15644 isolated from sewage inhibited MRSA isolates in vitro and in the murineskin infection model. Phage VB_SauS_SH-St 15644 belongs to Siphoviridae. The genome of the phage is a linear, 45,111 bp double-stranded DNA with GC content of 33.35%. Among the 37 clinical MRSA isolates tested, 12 (32%) were lysed by the phage in vitro. The phage was relatively stable at temperatures up to 40 °C or between pH 6 and 9. However, the phage was sensitive to UV light. 80% of the phage was approximately adsorbed to the host MRSA isolate in 4 min. The one-step growth curve showed that the latent period was about 12 min followed by the growth period (about 9 min). The burst size was estimated at 13 PFU per infected cell. Furthermore, in a murineskin infection model, the phage significantly inhibited MRSA infection. CONCLUSIONS: Our study suggested that phage VB_SauS_SH-St 15644 has a potential to inhibit MRSA skin infection.
Authors: Tingting Feng; Sebastian Leptihn; Ke Dong; Belinda Loh; Yan Zhang; Melanie I Stefan; Mingyue Li; Xiaokui Guo; Zelin Cui Journal: Front Microbiol Date: 2021-04-22 Impact factor: 5.640