Wang Song1,2,3, Yuan Yuan4, Naixin Yu5,3, Haike Gu6, Yunfeng Zhou5,3, Xigong Chen7, Shuaiyu Wang8, Kai Fan8, Zhengyan Ge9, Long Jin9, Yang Xu5,3. 1. College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China. 2. Research Center for Pharmacology and Toxicology, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. 3. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China. 4. College of veterinary medicine, China Agricultural University, Beijing 100083, China. 5. IMPLAD, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing100193, China. 6. Beijing Radiation Center, Beijing 100875, China. 7. Key Laboratory of Plant Resources Conservation and Utilization, College of Hunan Province, Jishou University, Jishou 416000, China. 8. College of veterinary medicine, China Agricultural University,Beijing 100083, China. 9. Experiment Research Center of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
Abstract
OBJECTIVE: To evaluate the antioxidant capacity of the extract from Jiangtang Xiaozhi recipe (JXR) of in vitro. METHODS: JXR extract was prepared according to previously reported method. In vitro antioxidant assays were used in this experiment, including 1,1-Diphenyl-2-picrylhydrazyl free radical (DPPH) radical scavenging ability, 2-2'-Azinobis-(3-ethylbenzthiazoline-6-sul phonate (ABTS) radical scavenging ability, reducing power assay, fluorescence recovery after photo bleaching assay, β-carotene bleaching assay, ferric thiocyanate assay, and thiobarbituric acid method. RESULTS: DPPH, ABTS assay showed that JXR extract had distinct effect on scavenging free radicals; reducing power and ferricreducing-antioxidant power assay showed that JXR extract possessed redox ability; β-Carotene bleaching assay and antioxidant activity in a linoleic acid system using ferric thiocyanate method, thiobarbituric acid assay indicated that JXR extract could effectively inhibit lipid peroxidation, and the effect was better than that of Vitamin C. CONCLUSION: JXR extract has significant antioxidant capacity in vitro.
OBJECTIVE: To evaluate the antioxidant capacity of the extract from Jiangtang Xiaozhi recipe (JXR) of in vitro. METHODS: JXR extract was prepared according to previously reported method. In vitro antioxidant assays were used in this experiment, including 1,1-Diphenyl-2-picrylhydrazyl free radical (DPPH) radical scavenging ability, 2-2'-Azinobis-(3-ethylbenzthiazoline-6-sul phonate (ABTS) radical scavenging ability, reducing power assay, fluorescence recovery after photo bleaching assay, β-carotene bleaching assay, ferric thiocyanate assay, and thiobarbituric acid method. RESULTS: DPPH, ABTS assay showed that JXR extract had distinct effect on scavenging free radicals; reducing power and ferricreducing-antioxidant power assay showed that JXR extract possessed redox ability; β-Carotene bleaching assay and antioxidant activity in a linoleic acid system using ferric thiocyanate method, thiobarbituric acid assay indicated that JXR extract could effectively inhibit lipid peroxidation, and the effect was better than that of Vitamin C. CONCLUSION: JXR extract has significant antioxidant capacity in vitro.