Yuanqing Cai1, Xinyu Fang2, Yang Chen3, Zida Huang4, Chaofan Zhang5, Wenbo Li6, Bin Yang7, Wenming Zhang8. 1. Department of Orthopedic Surgery, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China. Electronic address: yuanqing_cai@yeah.net. 2. Department of Orthopedic Surgery, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China. Electronic address: 421120540@qq.com. 3. Department of Orthopedic Surgery, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China. Electronic address: chenyang0596@163.com. 4. Department of Orthopedic Surgery, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China. Electronic address: huangzida@163.com. 5. Department of Orthopedic Surgery, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China. Electronic address: drcfzhang@gmail.com. 6. Department of Orthopedic Surgery, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China. Electronic address: liwenbo350001@163.com. 7. Department of Laboratory Medicine, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China. Electronic address: 18960963783@yeah.net. 8. Department of Orthopedic Surgery, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China. Electronic address: zhangwm0591@fjmu.edu.cn.
Abstract
OBJECTIVES: The purpose of this study was to explore the role of mNGS in the diagnosis of PJI by detecting the presence of bacteria in periprosthetic tissues. METHODS: Patients who were suspected of PJI and underwent surgery in our center were enrolled. Microbial culture and mNGS were performed on periprosthetic tissues collected intraoperatively. Patient age, body mass index (BMI), laboratory tests, microbial culture and mNGS results were recorded. The diagnostic performance of microbial culture and mNGS in the diagnosis of PJI was compared. RESULTS: A total of 44 patients were included. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy of periprosthetic tissue microbial culture in the diagnosis of PJI were 72.72%, 77.27%, 76.19%, 73.91% and 75%, respectively. The sensitivity, specificity, PPV, NPV and accuracy of periprosthetic tissue mNGS in the diagnosis of PJI were 95.45%, 90.91%, 91.3%, 95.24% and 93.18%, respectively. The sensitivity and specificity of mNGS in the diagnosis of PJI were superior to those of microbial culture. CONCLUSION: Periprosthetic tissue can be used as an alternative to synovial fluid and sonication fluid, as mNGS can improve the diagnosis of PJI. mNGS of the periprosthetic tissues showed higher sensitivity than microbial culture.
OBJECTIVES: The purpose of this study was to explore the role of mNGS in the diagnosis of PJI by detecting the presence of bacteria in periprosthetic tissues. METHODS:Patients who were suspected of PJI and underwent surgery in our center were enrolled. Microbial culture and mNGS were performed on periprosthetic tissues collected intraoperatively. Patient age, body mass index (BMI), laboratory tests, microbial culture and mNGS results were recorded. The diagnostic performance of microbial culture and mNGS in the diagnosis of PJI was compared. RESULTS: A total of 44 patients were included. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy of periprosthetic tissue microbial culture in the diagnosis of PJI were 72.72%, 77.27%, 76.19%, 73.91% and 75%, respectively. The sensitivity, specificity, PPV, NPV and accuracy of periprosthetic tissue mNGS in the diagnosis of PJI were 95.45%, 90.91%, 91.3%, 95.24% and 93.18%, respectively. The sensitivity and specificity of mNGS in the diagnosis of PJI were superior to those of microbial culture. CONCLUSION: Periprosthetic tissue can be used as an alternative to synovial fluid and sonication fluid, as mNGS can improve the diagnosis of PJI. mNGS of the periprosthetic tissues showed higher sensitivity than microbial culture.
Authors: Jun Tan; Yang Liu; Sabrina Ehnert; Andreas K Nüssler; Yang Yu; Jianzhong Xu; Tao Chen Journal: Front Cell Infect Microbiol Date: 2022-06-10 Impact factor: 6.073
Authors: Michael Riste; Pretin Davda; E Grace Smith; David H Wyllie; Martin Dedicoat; Simantini Jog; Steven Laird; Gerald Langman; Neil Jenkins; Jonathan Stevenson; Matthew K O'Shea Journal: BMC Infect Dis Date: 2021-02-05 Impact factor: 3.090