A S Muadica1, S Balasegaram2, K Beebeejaun2, P C Köster3, B Bailo3, M Hernández-de-Mingo3, A Dashti3, E Dacal3, J M Saugar3, I Fuentes3, D Carmena4. 1. Parasitology Reference and Research Laboratory, National Centre for Microbiology, Majadahonda, Madrid, Spain; Departamento de Ciências e Tecnologia, Universidade Licungo, Quelimane, Zambézia, Mozambique. 2. Field Epidemiology Services, National Infection Service, Public Health England, London, UK. 3. Parasitology Reference and Research Laboratory, National Centre for Microbiology, Majadahonda, Madrid, Spain. 4. Parasitology Reference and Research Laboratory, National Centre for Microbiology, Majadahonda, Madrid, Spain. Electronic address: dacarmena@isciii.es.
Abstract
OBJECTIVES: Chronic infections by enteric parasites including protist and helminthic species produce long-term sequelae on the health status of infected children. This study assesses potential associations linked with enteric parasite infections in symptomatic and asymptomatic children in Zambézia province, Mozambique. METHODS: In this prospective cross-sectional study, stool samples and epidemiological questionnaires on demographics and risk associations were collected from symptomatic children (n = 286) from clinical settings and asymptomatic (n = 807) children from 17 schools and creches aged 3‒14 years. We detected enteric parasites using PCR-based methods. We calculated prevalence (adjusted for age, sex, house construction, drinking water, and latrine use) and odds ratios (ORs) for risk associations with logistic regression, after adjusting for district, neighbourhood and symptoms. RESULTS: Numbers and adjusted prevalence (95% confidence intervals in parentheses) for the symptomatic and asymptomatic populations were Giardia duodenalis 120, 52% (22-82), 339, 42% (25-59); followed by Strongyloides stercoralis 52, 14% (9‒20), 180, 20% (15-25). Risk associations for G. duodenalis included drinking untreated river/spring water, OR 2.91 (1.80-4.70); contact with ducks, OR 14.96 (2.93‒76.31); dogs, OR 1.92 (1.04-3.52); cats, OR 1.73 (1.16-2.59), and a relative with diarrhoea, OR 2.59 (1.54‒4.37). Risk associations for S. stercoralis included having no latrine, OR 2.41 (1.44-4.02); drinking well water, OR 1.82 (1.02-3.25), and increasing age, OR 1.11 (1.04-1.20). CONCLUSIONS: We found a high prevalence of intestinal parasites regardless of the children's symptoms. Drinking well or river water, domestic animals, and latrine absence were contributing factors of human infections.
OBJECTIVES:Chronic infections by enteric parasites including protist and helminthic species produce long-term sequelae on the health status of infectedchildren. This study assesses potential associations linked with enteric parasite infections in symptomatic and asymptomatic children in Zambézia province, Mozambique. METHODS: In this prospective cross-sectional study, stool samples and epidemiological questionnaires on demographics and risk associations were collected from symptomatic children (n = 286) from clinical settings and asymptomatic (n = 807) children from 17 schools and creches aged 3‒14 years. We detected enteric parasites using PCR-based methods. We calculated prevalence (adjusted for age, sex, house construction, drinking water, and latrine use) and odds ratios (ORs) for risk associations with logistic regression, after adjusting for district, neighbourhood and symptoms. RESULTS: Numbers and adjusted prevalence (95% confidence intervals in parentheses) for the symptomatic and asymptomatic populations were Giardia duodenalis 120, 52% (22-82), 339, 42% (25-59); followed by Strongyloides stercoralis 52, 14% (9‒20), 180, 20% (15-25). Risk associations for G. duodenalis included drinking untreated river/spring water, OR 2.91 (1.80-4.70); contact with ducks, OR 14.96 (2.93‒76.31); dogs, OR 1.92 (1.04-3.52); cats, OR 1.73 (1.16-2.59), and a relative with diarrhoea, OR 2.59 (1.54‒4.37). Risk associations for S. stercoralis included having no latrine, OR 2.41 (1.44-4.02); drinking well water, OR 1.82 (1.02-3.25), and increasing age, OR 1.11 (1.04-1.20). CONCLUSIONS: We found a high prevalence of intestinal parasites regardless of the children's symptoms. Drinking well or river water, domestic animals, and latrine absence were contributing factors of humaninfections.
Authors: Augusto Messa; Pamela C Köster; Marcelino Garrine; Carol Gilchrist; Luther A Bartelt; Tacilta Nhampossa; Sérgio Massora; Karen Kotloff; Myron M Levine; Pedro L Alonso; David Carmena; Inácio Mandomando Journal: PLoS Negl Trop Dis Date: 2021-01-19
Authors: Fernando Salvador; Beatriz Lobo; Lidia Goterris; Carmen Alonso-Cotoner; Javier Santos; Elena Sulleiro; Begoña Bailo; David Carmena; Adrián Sánchez-Montalvá; Pau Bosch-Nicolau; Juan Espinosa-Pereiro; Isabel Fuentes; Israel Molina Journal: Biology (Basel) Date: 2021-04-19