Sameera A Gunawardena1, Jayani Wathsala Gunawardana2, Rohana Chandrajith3, Tharanga Thoradeniya4, Saroj Jayasinghe5. 1. Department of Forensic Medicine and Toxicology, Faculty of Medicine, University of Colombo, Kynsey Road, Colombo 08, Sri Lanka. Electronic address: sameera@fortox.cmb.ac.lk. 2. Department of Forensic Medicine and Toxicology, Faculty of Medicine, University of Colombo, Kynsey Road, Colombo 08, Sri Lanka. 3. Department of Geology, Faculty of Science, University of Peradeniya, Peradeniya, Sri Lanka. 4. Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Colombo, Kynsey Road, Colombo 08, Sri Lanka. 5. Department of Clinical Medicine, Faculty of Medicine, University of Colombo, Kynsey Road, Colombo 08, Sri Lanka.
Abstract
INTRODUCTION: Environmental pollution, especially by toxic trace elements, is a global health concern. Heavy metals such as Cadmium (Cd), Arsenic (As) and Lead (Pb) are associated with numerous disorders and are considered by some as an aetiological factor for the Chronic Kidney Disease (CKDu1) epidemic in Sri Lanka. This study explores patterns of bioaccumulation of six trace elements in kidneys obtained during forensic autopsies from urban and rural regions in Sri Lanka. METHODS: Kidney samples obtained from one urban district (n = 13) and three rural districts (n = 18) were lyophilized, microwave digested and profiled by ICP-MS techniques. RESULTS AND DISCUSSION: The mean age of the sampled population was 47.9 ± 11.3 yrs. Median (IQR) for Cd, As, Pb, Cr, Zn and Se were, 14.67(8.04-22.47) μg/g, 0.44(0.29-0.56) μg/g, 0.11(0.07-0.30) μg/g, 0.15(0.1096-0.3274), 25.55(17.24-39.35) μg/g and 0.52(0.37-0.84) μg/g, respectively. Cd, Zn and Se levels were significantly higher (p < 0.05) among the urban samples compared to that of the rural group. Zn and Se levels were higher among younger age groups. As, Pb and Cr did not show any significant differences between the two cohorts nor any correlations with age. CONCLUSION: This population-specific baseline study provides an insight into the differences in exposure to toxic trace elements and essential elements between urban and rural populations. Residents in CKDu affected rural districts did not appear to be at risk of toxic heavy metal exposure, however their renal bioaccumulation of nephroprotective essential elements was lower than urban residents.
INTRODUCTION: Environmental pollution, especially by toxic trace elements, is a global health concern. Heavy metals such asCadmium (Cd), Arsenic (As) and Lead (Pb) are associated with numerous disorders and are considered by some as an aetiological factor for the Chronic Kidney Disease (CKDu1) epidemic in Sri Lanka. This study explores patterns of bioaccumulation of six trace elements in kidneys obtained during forensic autopsies from urban and rural regions in Sri Lanka. METHODS: Kidney samples obtained from one urban district (n = 13) and three rural districts (n = 18) were lyophilized, microwave digested and profiled by ICP-MS techniques. RESULTS AND DISCUSSION: The mean age of the sampled population was 47.9 ± 11.3 yrs. Median (IQR) for Cd, As, Pb, Cr, Zn and Se were, 14.67(8.04-22.47) μg/g, 0.44(0.29-0.56) μg/g, 0.11(0.07-0.30) μg/g, 0.15(0.1096-0.3274), 25.55(17.24-39.35) μg/g and 0.52(0.37-0.84) μg/g, respectively. Cd, Zn and Se levels were significantly higher (p < 0.05) among the urban samples compared to that of the rural group. Zn and Se levels were higher among younger age groups. As, Pb and Cr did not show any significant differences between the two cohorts nor any correlations with age. CONCLUSION: This population-specific baseline study provides an insight into the differences in exposure to toxic trace elements and essential elements between urban and rural populations. Residents in CKDu affected rural districts did not appear to be at risk of toxic heavy metal exposure, however their renal bioaccumulation of nephroprotective essential elements was lower than urban residents.
Keywords:
Chronic kidney disease of uncertain etiology; Environmental pollution; Heavy metal bioaccumulation; Post mortem toxicological analysis; Toxic nephropathy