Nanami Senoo1,2, Noriyuki Miyoshi3, Eri Kobayashi1, Akihito Morita1, Jun Tanihata4,5, Shin'ichi Takeda4, Shinji Miura1. 1. Laboratory of Nutritional Biochemistry, Graduate School of Nutritional and Environmental Sciences, Shizuoka, Japan. 2. Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. 3. Laboratory of Biochemistry, Graduate School of Nutritional and Environmental Sciences, Shizuoka, Japan. 4. Department of Molecular Therapy, National Institute of Neuroscience, Tokyo, Japan. 5. Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan.
Abstract
INTRODUCTION: Phospholipids are essential components of cellular membranes and are closely associated with cellular functions; however, relationships involving skeletal muscle phospholipid profiles and their physiological phenotypes have been unclear. METHODS: We carried out comprehensive phospholipid analyses using liquid chromatography-tandem mass spectrometry to determine the phospholipid profiles of skeletal muscles derived from muscle-wasting mouse models including denervation and Duchenne muscular dystrophy mouse models (mdx), as well as rescued mdx mice expressing truncated dystrophin. RESULTS: Consistent phosphatidylcholine and phosphatidylethanolamine alterations in skeletal muscles isolated from denervation and mdx mice were observed. Notably, the levels of these phospholipids binding polyunsaturated fatty acids were reduced in denervated and mdx muscles. Moreover, rescuing the mdx pathology by expressing truncated dystrophin led to the restoration of phospholipid profiles. DISCUSSION: This study supports the hypothesis that phospholipid profiles of the skeletal muscle may be associated with skeletal muscle function. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
INTRODUCTION:Phospholipids are essential components of cellular membranes and are closely associated with cellular functions; however, relationships involving skeletal muscle phospholipid profiles and their physiological phenotypes have been unclear. METHODS: We carried out comprehensive phospholipid analyses using liquid chromatography-tandem mass spectrometry to determine the phospholipid profiles of skeletal muscles derived from muscle-wastingmouse models including denervation and Duchenne muscular dystrophymouse models (mdx), as well as rescued mdx mice expressing truncated dystrophin. RESULTS: Consistent phosphatidylcholine and phosphatidylethanolamine alterations in skeletal muscles isolated from denervation and mdx mice were observed. Notably, the levels of these phospholipids binding polyunsaturated fatty acids were reduced in denervated and mdx muscles. Moreover, rescuing the mdx pathology by expressing truncated dystrophin led to the restoration of phospholipid profiles. DISCUSSION: This study supports the hypothesis that phospholipid profiles of the skeletal muscle may be associated with skeletal muscle function. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
Authors: Patrick J Ferrara; Anthony R P Verkerke; J Alan Maschek; Justin L Shahtout; Piyarat Siripoksup; Hiroaki Eshima; Jordan M Johnson; Jonathan J Petrocelli; Ziad S Mahmassani; Thomas D Green; Joseph M McClung; James E Cox; Micah J Drummond; Katsuhiko Funai Journal: FASEB J Date: 2021-10 Impact factor: 5.834