Literature DB >> 32490096

Leaf tissue specific transcriptome sequence and de novo assembly datasets of Asiatic mangrove Rhizophora mucronata Lam.

S P Meera1, Anusha Sreeshan1, Anu Augustine1.   

Abstract

Transcriptome data is beneficial to explore molecular mechanisms of extreme adaptations in non- model organisms like mangroves. In this data article, five major datasets and two data sub sets of a salt secreting mangrove, Rhizophora mucronata Lam. were described. A combination of Illumina HiSeq 2500, Trinity, BLAST X, Bowtie 2 and BLAST 2GO was used for RNA Seq, de novo assembly, transcript annotation, gene expression estimation and gene ontology annotation respectively. The RNA Sequence (Read 1 and Read 2) in Sequence Read Archive amounting to 46,366,348 paired end raw reads is the first data set made open for de novo or comparative transcript assembly. Assembled sequences of 93960 gene transcripts constitute the second data set in Transcriptome Shotgun Assembly. The gene/protein annotations to the assembled transcripts give two sub data sets containing 93960 each of GenBank and GenPept entries with comprehensive cDNA and translated protein sequences of genes. Of these, predicted proteins for 87768 coding sequences, mapped to UniProtKB serve as the third data set. The gene expression levels of the annotated transcripts comprise the fourth data set in Gene Expression Omnibus. The fifth data set in Figshare includes 44,028 gene ontology terms extracted for 21,073 confident transcripts. The data sets provide a valuable resource for further analyses including transcriptomic changes in response to environmental stresses.
© 2020 Published by Elsevier Inc.

Entities:  

Keywords:  Annotation; De novo assembly; Mangrove; Rhizophora mucronata; salt tolerance; transcriptome

Year:  2020        PMID: 32490096      PMCID: PMC7262405          DOI: 10.1016/j.dib.2020.105747

Source DB:  PubMed          Journal:  Data Brief        ISSN: 2352-3409


  7 in total

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Journal:  Nucleic Acids Res       Date:  2002-01-01       Impact factor: 16.971

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Authors:  Jochen B W Wolf
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Authors:  Ben Langmead; Cole Trapnell; Mihai Pop; Steven L Salzberg
Journal:  Genome Biol       Date:  2009-03-04       Impact factor: 13.583

5.  De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis.

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Journal:  Nat Protoc       Date:  2013-07-11       Impact factor: 13.491

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Authors:  Manfred G Grabherr; Brian J Haas; Moran Yassour; Joshua Z Levin; Dawn A Thompson; Ido Amit; Xian Adiconis; Lin Fan; Raktima Raychowdhury; Qiandong Zeng; Zehua Chen; Evan Mauceli; Nir Hacohen; Andreas Gnirke; Nicholas Rhind; Federica di Palma; Bruce W Birren; Chad Nusbaum; Kerstin Lindblad-Toh; Nir Friedman; Aviv Regev
Journal:  Nat Biotechnol       Date:  2011-05-15       Impact factor: 54.908

7.  High-throughput functional annotation and data mining with the Blast2GO suite.

Authors:  Stefan Götz; Juan Miguel García-Gómez; Javier Terol; Tim D Williams; Shivashankar H Nagaraj; María José Nueda; Montserrat Robles; Manuel Talón; Joaquín Dopazo; Ana Conesa
Journal:  Nucleic Acids Res       Date:  2008-04-29       Impact factor: 16.971

  7 in total

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