Sachiko Inubushi1,2,3, Hiroki Kawaguchi1, Sachiko Mizumoto4, Tomonari Kunihisa4, Motoi Baba4, Yukiya Kitayama5, Toshifumi Takeuchi5, Robert M Hoffman2,3, Ryohei Sasaki6. 1. Division of Radiation Oncology, Kobe University Graduate School of Medicine, Hyogo, Japan. 2. AntiCancer Inc, San Diego, CA, U.S.A. 3. Department of Surgery, University of California, San Diego, CA, U.S.A. 4. Division of Breast and Endocrine Surgery Kobe University Graduate School of Medicine, Hyogo, Japan. 5. Graduate School of Engineering, Medical Device Fabrication Engineering Center, Graduate School of Engineering, Kobe University, Hyogo, Japan. 6. Division of Radiation Oncology, Kobe University Graduate School of Medicine, Hyogo, Japan yuunasasaki@hotmail.com inubushi0922@gmail.com.
Abstract
BACKGROUND/AIM: Exosomes are produced by normal and cancer cells. Exosomes are found in the serum of cancer patients and have been used for diagnosis and prognosis. Recently tears from non-cancer patients have been found to contain exosomes. In the present report we describe tears from advanced breast-cancer patients. MATERIALS AND METHODS: We found oncogenic miRNAs in the exosomes isolated from tear fluids obtained from five patients with metastatic breast cancer and compared them with tear exosomes form eight healthy volunteers. RESULTS: Tear exosomes had a significantly higher quantity of exosome markers than serum exosomes (CD9, CD63). Tear exosomes were subjected to quantitative reverse-transcription polymerase reaction (qRT-PCR), and western blot analysis to elucidate the status of miRNAs, previously reported in serum from patients with metastatic breast cancer. qRT-PCR and western-blot analysis revealed that breast-cancer-specific miR-21 and miR-200c were highly expressed in tear exosomes from metastatic breast cancer patients in contrast to tear exosomes from healthy volunteers. CONCLUSION: Tear exosomes can be a potential source of diagnostic and prognostic biomarkers for metastatic breast cancer, and possibly other cancers or diseases. Copyright
BACKGROUND/AIM: Exosomes are produced by normal and cancer cells. Exosomes are found in the serum of cancerpatients and have been used for diagnosis and prognosis. Recently tears from non-cancerpatients have been found to contain exosomes. In the present report we describe tears from advanced breast-cancerpatients. MATERIALS AND METHODS: We found oncogenic miRNAs in the exosomes isolated from tear fluids obtained from five patients with metastatic breast cancer and compared them with tear exosomes form eight healthy volunteers. RESULTS: Tear exosomes had a significantly higher quantity of exosome markers than serum exosomes (CD9, CD63). Tear exosomes were subjected to quantitative reverse-transcription polymerase reaction (qRT-PCR), and western blot analysis to elucidate the status of miRNAs, previously reported in serum from patients with metastatic breast cancer. qRT-PCR and western-blot analysis revealed that breast-cancer-specific miR-21 and miR-200c were highly expressed in tear exosomes from metastatic breast cancerpatients in contrast to tear exosomes from healthy volunteers. CONCLUSION: Tear exosomes can be a potential source of diagnostic and prognostic biomarkers for metastatic breast cancer, and possibly other cancers or diseases. Copyright
Authors: Amal F Gharib; Amany Salah Khalifa; Emad Mohamed Eed; Hamsa Jameel Banjer; Ashjan Ali Shami; Ahmad El Askary; Wael H Elsawy Journal: In Vivo Date: 2022 May-Jun Impact factor: 2.406