Preparation and characterization of abalone shells derived biological mesoporous hydroxyapatite microspheres for drug delivery.

The rapid growth of the abalone industry has brought a great burden to the environment because of their inedible shells. Aiming at environmental and resource sustainability, porous microspheres of carbonate-substituted hydroxyapatite (HAP) were prepared by a hydrothermal method using abalone shells; then, they were further used as a carrier for doxorubicin (DOX) in a drug delivery system. The porous HAP microspheres were approximately 6 μm in size with a considerable specific surface area and average pore size (128.6659 cm2/g and 9.064 nm, respectively), which ensured excellent drug-handling capacity (95.542%). In addition, the pH responsiveness of the drug release system was favorable for effective in vivo drug release in an acidic tumor microenvironment. Moreover, the drug-loaded microspheres could effectively induce apoptosis of MCF-7 cells but were less cytotoxic to MC3T3-E1 cells. Because of its good biocompatibility, high drug loading capacity and controlled drug release property, the porous microspheres prepared in this experiment have potential application value in drug delivery and tumor therapy; furthermore, they make full use of abalone shells, providing environmental sustainability.