Zahra Aramesh-Boroujeni1, Shohreh Jahani2, Mozhgan Khorasani-Motlagh3, Kagan Kerman4, Meissam Noroozifar5. 1. Isfahan University of Medical Sciences, Isfahan, Iran; Department of Chemistry, University of Sistan and Baluchestan, Zahedan, 98135-674, Iran. Electronic address: zaramesh.boroujeni@gmail.com. 2. Nano Bioeletrochemistry Research Center, Bam University of Medical Sciences, Bam, Iran. 3. Department of Chemistry, University of Sistan and Baluchestan, Zahedan, 98135-674, Iran. 4. Department of Physical and Environmental Sciences, University of Toronto Scarborough 1265 Military Trail, Toronto, Ontario, M1C 1A4, Canada. 5. Department of Physical and Environmental Sciences, University of Toronto Scarborough 1265 Military Trail, Toronto, Ontario, M1C 1A4, Canada. Electronic address: m.noroozifar@utoronto.ca.
Abstract
BACKGROUND: There is a crucial need for finding and developing new compounds as the anticancer and antimicrobial agents with better activity, specific target, and less toxic side effects. OBJECTIVES: Base on the potential anticancer properties of lanthanide complexes, in the paper, the biological applications of terbium (Tb) complex, containing 2,9-dimethyl- 1,10-phenanthroline (Me2Phen) such as anticancer, antimicrobial, DNA cleavage ability, the interaction with FS-DNA (Fish-Salmon DNA) and BSA (Bovine Serum Albumin) was examined. METHODS: The interaction of Tb-complex with BSA and DNA was studied by emission spectroscopy, absorption titration, viscosity measurement, CD spectroscopy, competitive experiments, and docking calculation. Also, the ability of this complex to cleave DNA was reported by gel electrophoresis. Tb-complex was concurrently screened for its antibacterial activities by different methods. Besides, the nanocarriers of Tb-complex (lipid nanoencapsulation (LNEP) and the starch nanoencapsulation (SNEP)), as active anticancer candidates, were prepared. MTT technique was applied to measure the antitumor properties of these compounds on human cancer cell lines. RESULTS: The experimental and docking results suggest significant binding between DNA as well as BSA with terbium-complex. Besides, groove binding plays the main role in the binding of this compound with DNA and BSA. The competitive experiment with hemin demonstrated that the terbium complex was bound at site III of BSA, which was confirmed by the docking study. Also, Tb-complex was concurrently screened for its DNA cleavage, antimicrobial, and anticancer activities. The anticancer properties of LNEP and SNEP are more than the terbium compound. CONCLUSIONS: Tb-complex can bond to DNA/BSA with high binding affinity. Base on biological applications of Tb-complex, it can be concluded that this complex and its nanocarriers can suggest as novel anticancer, antimicrobial candidates.
BACKGROUND: There is a crucial need for finding and developing new compounds as the anticancer and antimicrobial agents with better activity, specific target, and less toxic side effects. OBJECTIVES: Base on the potential anticancer properties of lanthanide complexes, in the paper, the biological applications of terbium (Tb) complex, containing 2,9-dimethyl- 1,10-phenanthroline (Me2Phen) such as anticancer, antimicrobial, DNA cleavage ability, the interaction with FS-DNA (Fish-Salmon DNA) and BSA (Bovine Serum Albumin) was examined. METHODS: The interaction of Tb-complex with BSA and DNA was studied by emission spectroscopy, absorption titration, viscosity measurement, CD spectroscopy, competitive experiments, and docking calculation. Also, the ability of this complex to cleave DNA was reported by gel electrophoresis. Tb-complex was concurrently screened for its antibacterial activities by different methods. Besides, the nanocarriers of Tb-complex (lipid nanoencapsulation (LNEP) and the starch nanoencapsulation (SNEP)), as active anticancer candidates, were prepared. MTT technique was applied to measure the antitumor properties of these compounds on humancancer cell lines. RESULTS: The experimental and docking results suggest significant binding between DNA as well as BSA with terbium-complex. Besides, groove binding plays the main role in the binding of this compound with DNA and BSA. The competitive experiment with hemin demonstrated that the terbium complex was bound at site III of BSA, which was confirmed by the docking study. Also, Tb-complex was concurrently screened for its DNA cleavage, antimicrobial, and anticancer activities. The anticancer properties of LNEP and SNEP are more than the terbium compound. CONCLUSIONS: Tb-complex can bond to DNA/BSA with high binding affinity. Base on biological applications of Tb-complex, it can be concluded that this complex and its nanocarriers can suggest as novel anticancer, antimicrobial candidates.
Authors: Mohammad Mehdi Moarefdoust; Shohreh Jahani; Mehran Moradalizadeh; Mohammad Mehdi Motaghi; Mohammad Mehdi Foroughi Journal: ChemistryOpen Date: 2022-03 Impact factor: 2.911