Getúlio P de Oliveira1,2, William F Porto3, Cintia C Palu4,5, Lydyane M Pereira6, Alessandra M M Reis6, Tatiana G Marçola7, Antonio R Teixeira-Neto7, Octavio L Franco1,3,6,8, Rinaldo W Pereira1,6,8. 1. Programa de pós-graduação em Patologia Molecular, Universidade de Brasília-UnB, Campus Universitário Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil. 2. Division of Allergy and Inflammation, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA. 3. Programa de pós-graduação em Biotecnologia, S-Inova Biotech, Universidade Católica Dom Bosco-UCDB, Campo Grande, MS, Brasil. 4. NSilico Life Science LTDA, Unit 1.23, Nova Center, Belfield Innovation Park, Dublin, Ireland. 5. University College Cork, Cork, Ireland. 6. Programa de pós-graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília-UCB, Brasília, DF, Brasil. 7. Programa de pós-graduação em Saúde Animal, Universidade de Brasília-UnB, Campus Universitário Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil. 8. Programa de pós-graduação em Educação Física, Universidade Católica de Brasília-UCB, Brasília, DF, Brasil.
Abstract
BACKGROUND: Physical exercise is an essential factor in preventing and treating metabolic diseases by promoting systemic benefits throughout the body. The molecular factors involved in this process are poorly understood. Micro RNAs (miRNAs) are small non-coding RNAs that inhibit mRNA transcription. MiRNAs, which can participate in the benefits of exercise to health, circulate in plasma in extracellular particles (EP). Horses that undergo endurance racing are an excellent model to study the impact of long-duration/low intensity exercise in plasma EP miRNAs. OBJECTIVES: To evaluate the effects of 160 km endurance racing on horse plasma extracellular particles and their miRNA population. STUDY DESIGN: Cohort study. METHODS: We collected plasma from five Arabian horses during five time-points of an endurance ride. Extracellular particles were purified from plasma and characterised by electron microscopy, resistive pulse sensing (qNano) and western blotting. Small RNAs were purified from horse plasma EP, and sequencing was performed. RESULTS: Endurance racing increased EP concentration and average diameter compared to before the race. Western blotting showed a high concentration of extracellular vesicles proteins 2 hours after the race, which returned to baseline 15 hours after the race. MicroRNA differential expression analysis revealed increasing levels of eca-miR-486-5p during and after the race, and decreasing levels of eca-miR-9083 after the end. CONCLUSIONS: This study adds new data about the variation in plasma EP concentrations after long-distance exercise and brings new insights about the roles of exercise-derived EP miRNAs during low-intensity endurance exercise.
BACKGROUND: Physical exercise is an essential factor in preventing and treating metabolic diseases by promoting systemic benefits throughout the body. The molecular factors involved in this process are poorly understood. Micro RNAs (miRNAs) are small non-coding RNAs that inhibit mRNA transcription. MiRNAs, which can participate in the benefits of exercise to health, circulate in plasma in extracellular particles (EP). Horses that undergo endurance racing are an excellent model to study the impact of long-duration/low intensity exercise in plasma EP miRNAs. OBJECTIVES: To evaluate the effects of 160 km endurance racing on horse plasma extracellular particles and their miRNA population. STUDY DESIGN: Cohort study. METHODS: We collected plasma from five Arabian horses during five time-points of an endurance ride. Extracellular particles were purified from plasma and characterised by electron microscopy, resistive pulse sensing (qNano) and western blotting. Small RNAs were purified from horse plasma EP, and sequencing was performed. RESULTS: Endurance racing increased EP concentration and average diameter compared to before the race. Western blotting showed a high concentration of extracellular vesicles proteins 2 hours after the race, which returned to baseline 15 hours after the race. MicroRNA differential expression analysis revealed increasing levels of eca-miR-486-5p during and after the race, and decreasing levels of eca-miR-9083 after the end. CONCLUSIONS: This study adds new data about the variation in plasma EP concentrations after long-distance exercise and brings new insights about the roles of exercise-derived EP miRNAs during low-intensity endurance exercise.