Mehdi Mirsaeidi1, Hesham R Omar2, Andrew Calzadilla1, Ahmad El Khatib1, Philip Whitney1, Nadera Sweiss3, Daniel Culver4, Michael Campos1, Robert Baughman5, Roberto Machado2. 1. Division of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of Miami, FL, USA. 2. Internal Medicine Department, Mercy Medical Center, Iowa, USA. 3. Division of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of Illinois at Chicago, IL, USA. 4. Department of Pulmonary, Allergy, and Critical Care Medicine, Cleveland Clinic, Cleveland, OH, USA. 5. Division of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of Cincinnati, OH, USA.
Abstract
Introduction: The pathogenesis of pulmonary fibrosis in sarcoidosis is not known. We hypothesized that higher levels of circulatory growth factors are present in early stages of pulmonary sarcoidosis and may be associated with pulmonary fibrosis. Methods: Age and sex-matched subjects with sarcoidosis stage 0-1 (n=18), stage 4-5 (n=13) and healthy controls (n=5) had their serum TGF-β1, FGF, and VEGF levels measured as well as their gene expressions determined in peripheral blood mononuclear cells. Results: TGF-β1 levels were significantly higher in patients with stage 0-1 sarcoidosis compared with normal healthy control patients (25,488 vs. 13800 pg/ml, P=0.05). Patients with sarcoidosis stage 4 had a 1.3-fold higher peripheral blood mononuclear cells (PBMC) gene expression of TGF-β1 compared with subjects at stage 0-1 (P= 0.041). The serum levels of FGF, and VEGF had a trend towards higher levels in sarcoidosis subjects compared to normal controls. Conclusion: These results suggest that cell growth factors levels are high in early stages of sarcoidosis. These findings should be validated in larger studies. (Sarcoidosis Vasc Diffuse Lung Dis 2018; 35: 213-217). Copyright:
Introduction: The pathogenesis of pulmonary fibrosis in sarcoidosis is not known. We hypothesized that higher levels of circulatory growth factors are present in early stages of pulmonary sarcoidosis and may be associated with pulmonary fibrosis. Methods: Age and sex-matched subjects with sarcoidosis stage 0-1 (n=18), stage 4-5 (n=13) and healthy controls (n=5) had their serum TGF-β1, FGF, and VEGF levels measured as well as their gene expressions determined in peripheral blood mononuclear cells. Results: TGF-β1 levels were significantly higher in patients with stage 0-1 sarcoidosis compared with normal healthy control patients (25,488 vs. 13800 pg/ml, P=0.05). Patients with sarcoidosis stage 4 had a 1.3-fold higher peripheral blood mononuclear cells (PBMC) gene expression of TGF-β1 compared with subjects at stage 0-1 (P= 0.041). The serum levels of FGF, and VEGF had a trend towards higher levels in sarcoidosis subjects compared to normal controls. Conclusion: These results suggest that cell growth factors levels are high in early stages of sarcoidosis. These findings should be validated in larger studies. (Sarcoidosis Vasc Diffuse Lung Dis 2018; 35: 213-217). Copyright:
Authors: Jon E Peterson; David Zurakowski; Joseph E Italiano; Lea V Michel; Susan Connors; Marsha Oenick; Robert J D'Amato; Giannoula L Klement; Judah Folkman Journal: Angiogenesis Date: 2012-03-09 Impact factor: 9.596
Authors: Mehdi Mirsaeidi; Mohammad Mehdi Banoei; Caleb K Nienow; Taimur Abassi; Anoushirvan Hakim; Dean Schraufnagel; Brent W Winston; Nadera Sweiss; Robert Baughman; Joe G N Garcia; Roberto Machado Journal: Sarcoidosis Vasc Diffuse Lung Dis Date: 2016-03-29 Impact factor: 0.670
Authors: Jon E Peterson; David Zurakowski; Joseph E Italiano; Lea V Michel; Lucius Fox; Giannoula L Klement; Judah Folkman Journal: Am J Hematol Date: 2010-07 Impact factor: 10.047
Authors: Donal J Sexton; M W O'Reilly; P Geoghegan; S M Kinsella; P J Moran; A W O'Regan Journal: Sarcoidosis Vasc Diffuse Lung Dis Date: 2016-08-01 Impact factor: 0.670
Authors: Katerina M Antoniou; Giannoula Soufla; Athanasia Proklou; George Margaritopoulos; Christiana Choulaki; Rena Lymbouridou; Katerina D Samara; Demetrios A Spandidos; Nikolaos M Siafakas Journal: Clin Dev Immunol Date: 2010-02-14
Authors: N Khalil; R N O'Connor; H W Unruh; P W Warren; K C Flanders; A Kemp; O H Bereznay; A H Greenberg Journal: Am J Respir Cell Mol Biol Date: 1991-08 Impact factor: 6.914