| Literature DB >> 32474857 |
Ao-Dun Tuoya1, Dong-Xue Wang2, Yu-Shu Xing3,4, Rui-Jun Liu1, Yu-Xia Hu3,4, Meng-di Zhang2,4, Tu-Ya Bai2,4, Xiao-Li Lv2,4, Jun Li4, Fu-Hou Chang5,6,7.
Abstract
In order to demonstrate the relationship between methylation of fragile histidine triad (FHIT) and T-cadherin/H-cadherin (CDH13) genes and liver cancer, the methylation status of FHIT and CDH13 was detected in healthy individuals and in Mongolian and Han patients with liver cancer. The phenol-chloroform method was used to extract genomic DNA. The methylation specific polymerase chain reaction method was applied to detect the methylation status of FHIT and CDH13. The relationship between smoking and alcohol consumption and gene (FHIT and CDH13) methylation was analyzed. There was significant difference in methylation rate of FHIT (72.67%, 34.67%) and CDH13 (72.0%, 28.0%) between liver cancer patients and healthy individuals of Mongolian descent (P<0.05), as well as that of FHIT (68%, 30.67%) and CDH13 (64%, 26%) between liver cancer patients and healthy individuals of Han individuals (P<0.05). There was also a relationship between smoking and drinking and the methylation of FHIT and CDH13 (P<0.05). Thus, the methylation of FHIT and CDH13 had a relationship with liver cancer incidence. Smoking and alcohol ingestion may promote the methylation of FHIT and CDH13.Entities:
Keywords: T-cadherin/H-cadherin; fragile histidine triad; liver cancer; methylation; susceptibility
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Year: 2020 PMID： 32474857 DOI： 10.1007/s11596-020-2202-4
Source DB: PubMed Journal: Curr Med Sci ISSN： 2523-899X